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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-MUC16 antibody [OV185:1] (ab697)

  • Datasheet
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Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)

    Key features and details

    • Mouse monoclonal [OV185:1] to MUC16
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human MUC16 protein (ab164957)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Pair
    Product image
    Human GFAP Matched Antibody Pair Kit (ab222279)

    View more associated products

    Overview

    • Product name

      Anti-MUC16 antibody [OV185:1]
      See all MUC16 primary antibodies
    • Description

      Mouse monoclonal [OV185:1] to MUC16
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Human
      See all applications and species data
    • Immunogen

      Partially purified human mucin fraction from a pool of cancer tissues of patients with epithelial ovarian.

    • Positive control

      • Ovarian carcinoma.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.05% Sodium azide
      Constituent: 1% BSA
    • Concentration information loading...
    • Purity

      Tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      OV185:1
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Tags & Cell Markers
      • Cell Type Markers
      • Tumor Associated
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Proteins
      • MUC
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • MUC

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant Human MUC16 protein (ab164957)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab697 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Human
    Application Abreviews Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
    • Tissue specificity

      Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
    • Sequence similarities

      Contains 2 ANK repeats.
      Contains 56 SEA domains.
    • Domain

      Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
    • Post-translational
      modifications

      Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
      Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
      May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
      May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
    • Cellular localization

      Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
    • Target information above from: UniProt accession Q8WXI7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 94025 Human
      • Omim: 606154 Human
      • SwissProt: Q8WXI7 Human
      • Unigene: 432676 Human
      • Alternative names

        • CA 125 antibody
        • CA-125 antibody
        • CA125 antibody
        • CA125 ovarian cancer antigen antibody
        • Cancer antigen 125 antibody
        • FLJ14303 antibody
        • MUC 16 antibody
        • MUC-16 antibody
        • MUC16 antibody
        • MUC16_HUMAN antibody
        • Mucin 16 antibody
        • Mucin 16 cell surface associated antibody
        • Mucin-16 antibody
        • Mucin16 antibody
        • Ovarian cancer related tumor marker CA125 antibody
        • Ovarian cancer-related tumor marker CA125 antibody
        • Ovarian carcinoma antigen CA125 antibody
        see all

      Images

      • Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
        Flow Cytometry - Anti-MUC16 antibody [OV185:1] (ab697)
        Overlay histogram showing HeLa cells stained with ab697 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab697, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

        Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (2)

      Publishing research using ab697? Please let us know so that we can cite the reference in this datasheet.

      ab697 has been referenced in 2 publications.

      • Shibata S  et al. Selective Laminin-Directed Differentiation of Human Induced Pluripotent Stem Cells into Distinct Ocular Lineages. Cell Rep 25:1668-1679.e5 (2018). PubMed: 30404017
      • Kobayashi Y  et al. Generation of a TALEN-mediated, p63 knock-in in human induced pluripotent stem cells. Stem Cell Res 25:256-265 (2017). IF ; Human . PubMed: 29179035

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