Overview

  • Product name
    Anti-MUC16 antibody [X325]
    See all MUC16 primary antibodies
  • Description
    Mouse monoclonal [X325] to MUC16
  • Host species
    Mouse
  • Specificity
    Epitope specificity group B (ISOBM classification).
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human MUC16. Purified from human ovarian carcinoma.
    Database link: Q8WXI7

  • Positive control
    • IHC-P: Human lung, benign ovarian tumor and bladder carcinoma tissue.
  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

Applications

Our Abpromise guarantee covers the use of ab10033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately >1000 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
  • Tissue specificity
    Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
  • Sequence similarities
    Contains 2 ANK repeats.
    Contains 56 SEA domains.
  • Domain
    Composed of three domains, a Ser-, Thr-rich N-terminal domain, a repeated domain containing more than 60 partially conserved tandem repeats of 156 amino acids each (AAs 12061-21862) and a C-terminal transmembrane contain domain with a short cytoplasmic tail.
  • Post-translational
    modifications
    Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.
    Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.
    May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.
    May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
  • Cellular localization
    Cell membrane. Secreted > extracellular space. May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CA 125 antibody
    • CA-125 antibody
    • CA125 antibody
    • CA125 ovarian cancer antigen antibody
    • Cancer antigen 125 antibody
    • FLJ14303 antibody
    • MUC 16 antibody
    • MUC-16 antibody
    • MUC16 antibody
    • MUC16_HUMAN antibody
    • Mucin 16 antibody
    • Mucin 16 cell surface associated antibody
    • Mucin-16 antibody
    • Mucin16 antibody
    • Ovarian cancer related tumor marker CA125 antibody
    • Ovarian cancer-related tumor marker CA125 antibody
    • Ovarian carcinoma antigen CA125 antibody
    see all

Images

  • ab10033 staining MUC16 in human lung tissue (alveolocytes type II) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • ab10033 staining MUC16 in human benign ovarian tumor tissue (serous papillary cystadenoma) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

  • ab10033 staining MUC16 in human bladder carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Lee H  et al. DA-6034-induced mucin secretion via Ca2+-dependent pathways through P2Y receptor stimulation. Invest Ophthalmol Vis Sci 55:6565-74 (2014). Read more (PubMed: 25212776) »
  • Shimizu A  et al. Coexpression of MUC16 and mesothelin is related to the invasion process in pancreatic ductal adenocarcinoma. Cancer Sci 103:739-46 (2012). Human . Read more (PubMed: 22320398) »
See all 4 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cynomolgus Monkey Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Ventana CC1
Permeabilization
No
Specification
Tonsil
Blocking step
Dako protein block as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Fixative
Formaldehyde

Sheng Zhong

Verified customer

Submitted Nov 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (urinary tract)
Specification
urinary tract
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 24 2013

Answer

Thank you for contacting us.

Following Western blot conditions have been tested with our anti-CA-125 MAbs:

1. CA125 is immobilized on the nitrocellulose membrane for 30 min (400 mA, 25 mM Tris-Hcl, 192 mM Glycin, 0,1% SDS, 20% ethanol) (Deglycosylation of the CA125 is not needed)

2. Blocking by 0.2% casein

3. Incubation with diluted MAbs for 1 hour at + 20°C

4. Washing (PBS-Tween)

5. Incubation with conjugate for 1 hour at + 20°C

6. washing (PBS-Tween)

7. Staining by 0.05 % DAB, H2O2.

Tween is added to buffers in each step.

With crude antigen from cells as sample and MAb X75 a diffuse area on top of the gel around 200 kDa is detected. Unfortunately image is not available.

We have used 6% or 8% gel.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you very much for contacting us.

I just wanted to let you know that I am still working on your inquiry. I have contacted the originator of this product for the information you requested, and I am awaiting a reply.

I am very sorry for the delay. I will forward to you the information as soon as I receive it. Thank you for your patience.

Read More

Answer

Thank you for your resposne.

As my colleauge has explained in the previous e-mail, our antibodies and proteins will regrettably not have been tested and guaranteed in the application you are using (i.e. electrical biosensors).

As ELISA is the closest application, I am pleased to recommend some antibodies suitable for this applications, however these will not be covered by our guarantee for electrical biosensors.

CA 15.3 - none

TRU-QUANT - none

CA 27.29 - none

CA125 - ab10033, ab10029, ab19551

CEA (carcinoembryonic antigen) - ab15987, ab4451,



If you need any further assistance in the future, please do not hesitate to contact me.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (human colon and pancreas)
Total protein in input
5000 µg
Specification
human colon and pancreas
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Aug 02 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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