Overview

  • Product name

    Anti-MUC17 antibody
  • Description

    Rabbit polyclonal to MUC17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    antigen sequence, corresponding to amino acids 4122-4262 of Human MUC17.

  • Positive control

    • Human small intestine tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab122184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Probably plays a role in maintaining homeostasis on mucosal surfaces.
  • Tissue specificity

    Expressed almost exclusively in the intestine. Expression is especially high in both the duodenum and transverse colon. Expressed in mature absorptive cells of the small intestinal villi. No expression is detected in goblet cells. Highly expressed in pancreatic adenocarcinoma tissue (at protein level). Expression is not detectable in normal pancreas, in pancreatitis or in cell lines derived from other cancers.
  • Sequence similarities

    Contains 1 EGF-like domain.
    Contains 1 SEA domain.
  • Post-translational
    modifications

    Probably cleaved within the SEA domain.
    N-glycosylated. Contains high mannose and complex-type glycans. The forms containing the complex type glycans localize to the cell surface. Not O-glycosylated.
  • Cellular localization

    Cell membrane and Secreted. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Membrane mucin MUC17 antibody
    • MUC-17 antibody
    • MUC-3 antibody
    • MUC17 antibody
    • MUC17_HUMAN antibody
    • Mucin 17, cell surface associated antibody
    • Mucin-17 antibody
    • Secreted mucin MUC17 antibody
    • Small intestinal mucin MUC3 antibody
    • Small intestinal mucin-3 antibody
    see all

Images

  • ab122184 at 1/20 dilution staining MUC17 in Paraffin-embedded Human Small intestine tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Yang CW  et al. Genetic variations of MUC17 are associated with endometriosis development and related infertility. BMC Med Genet 16:60 (2015). IHC . Read more (PubMed: 26285705) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (MUC17 overexpressing HRK293T cells)
Permeabilization
No
Gating Strategy
singlets in viable cells
Specification
MUC17 overexpressing HRK293T cells
Preparation
Cell harvesting/tissue preparation method: cells detacted by Versene
Sample buffer: 2% FBS in PBS
Fixation
cells not fixed

Abcam user community

Verified customer

Submitted Feb 11 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (pancreactic cell lines)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
30 µg
Specification
pancreactic cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 11 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-7 breast Ca. adenocarcinoma)
Permeabilization
No
Specification
MCF-7 breast Ca. adenocarcinoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Waleed Amri

Verified customer

Submitted Apr 06 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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