• Product name

    Anti-MUC2 antibody [996/1]
    See all MUC2 primary antibodies
  • Description

    Mouse monoclonal [996/1] to MUC2
  • Host species

  • Specificity

    ab11197 recognises the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections ab11197 recognises colon, liver and prostate tissues strongly. The antibody recognises malignant colonic mucosa as well as normal mucosa.
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MUC2. Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163


    Database link: Q02817

  • Positive control

    • Normal colon
  • General notes

    We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.


    Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.



Our Abpromise guarantee covers the use of ab11197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20418870
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. This product does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.
WB Use at an assay dependent concentration. Predicted molecular weight: 621.5 kDa.


  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational

    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all


This product has been referenced in:

  • Tan HY  et al. A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies. PLoS One 13:e0197101 (2018). Read more (PubMed: 29746551) »
  • Wang YC  et al. Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study. Can J Gastroenterol Hepatol 2018:4149317 (2018). Read more (PubMed: 29785394) »
See all 21 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Human Purified protein (duodenal extracts)
duodenal extracts
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Aug 12 2019

Immunocytochemistry/ Immunofluorescence
Human Cell (Tumor Tissue)
Yes - 0.3% Triton
Tumor Tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C

Dr. Ashok kumar Dilly

Verified customer

Submitted Sep 20 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Mouse Tissue Section (Colon))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH6
Yes - Triton 0.2%/PBS
Mouse Tissue Section (Colon)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C

Ms. Claudia Champagne

Verified customer

Submitted Jun 22 2015

Immunocytochemistry/ Immunofluorescence
Mouse Cell (peritonei tumor, Epithelial cell type)
peritonei tumor, Epithelial cell type
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C

Abcam user community

Verified customer

Submitted May 07 2015


Thank you for your inquiry.

Unfortunately, we are not able to release the immunogen sequence for this particular antibody as it is proprietary information.

We aim to provide as much information as possible to our customers, so I am sorry that this has not been possible on this occasion.

Should you have any further questions, please do not hesitate to contact us.

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Thank you for contacting us.

According to our Technical FAQs, we would like to suggest to use 1 ug/ml as a starting concentration for this antibody.

Regarding the gel we would like suggest to use a gel between 5% and 7.5%. Please take care with the handling as they are very delicate and easy to rip.

In addition, here's a protocol for the transfer/blotting of this heavy protein:

1. Don't use PVDF membrane, nitrocellulose membrane works much better.
2. Use Tris-glycine based buffers.
3. Transfer should be longer and lower temperature (overnight at 4C and at 30V).
4. Use 2 pieces of Wattman for each part of the "sandwich".
5. Add some more SDS to the system than for normal standard WB, add 2 ml of 10% SDS to 2 l of transfer buffer which is Tris based.

Transfer Buffer:
39 mM Glycine
48 mM Tris Base
0.037% SDS (electrophoresis grade)
20% Methanol

In addition, a wet transfer may be more sufficient than a semi-dry.

Finally, please see the reference we have on our website under"Specific References":

Guilmeau S et al. Heterogeneity of Jagged1 expression in human and mouse intestinal tumors: implications for targeting Notch signaling. Oncogene : (2009). WB; Mouse.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Thank you for contacting Abcam.
We have not specifically tested this antibody on immature and mature mucin. However, I have done some research. There's a paper which looks at O-glycosylation of MUC2. Specifically, this paper notices aberrant glycosylation of MUC-2 in diseased tissue. Because this antibody recognizes both malignant and normal mucosa and the O-glycosylation differs depending on the disease state, it is unlikely that the glycosylation state of the protein has an effect on antibody recognition.
I hope this helps, but please let me know if you have any follow up questions.

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