Key features and details
- Mouse monoclonal [996/1] to MUC2
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MUC2 antibody [996/1]
See all MUC2 primary antibodies
DescriptionMouse monoclonal [996/1] to MUC2
Specificityab11197 recognises the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections ab11197 recognises colon, liver and prostate tissues strongly. The antibody recognises malignant colonic mucosa as well as normal mucosa.
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MUC2. Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163
TPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTT
Database link: Q02817
- Normal colon
We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography from tissue culture supernatant.
FunctionCoats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Tissue specificityColon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
Cellular localizationSecreted. In the intestine, secreted into the inner and outer mucus layers.
- Information by UniProt
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
ab11197 has been referenced in 27 publications.
- Wang Y et al. Single-cell transcriptome analysis reveals differential nutrient absorption functions in human intestine. J Exp Med 217:N/A (2020). PubMed: 31753849
- Cantero-Recasens G et al. Sodium channel TRPM4 and sodium/calcium exchangers (NCX) cooperate in the control of Ca2+-induced mucin secretion from goblet cells. J Biol Chem 294:816-826 (2019). PubMed: 30482841
- In JG et al. Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium. J Vis Exp N/A:N/A (2019). PubMed: 31033964
- Parker RN et al. Design of Silk-Elastin-Like Protein Nanoparticle Systems with Mucoadhesive Properties. J Funct Biomater 10:N/A (2019). PubMed: 31726786
- Janardhan KS et al. Immunohistochemistry in Investigative and Toxicologic Pathology. Toxicol Pathol 46:488-510 (2018). PubMed: 29966501