Key features and details
- Mouse monoclonal [996/1] to MUC2
- Suitable for: Flow Cyt, IHC-Fr, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MUC2 antibody [996/1]
See all MUC2 primary antibodies
DescriptionMouse monoclonal [996/1] to MUC2
Specificityab11197 recognises the human MUC2 mucin, and shows no cross-reactivity with MUC1, MUC3 or MUC4 mucins. In tissue sections ab11197 recognises colon, liver and prostate tissues strongly. The antibody recognises malignant colonic mucosa as well as normal mucosa.
Tested applicationsSuitable for: Flow Cyt, IHC-Fr, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MUC2. Synthetic peptide: TPTPTGTQTP TTTPITTTTT VTPTPTPTGT QTPTTTPITT T This is from the tandem repeat are of the sequence and can be found starting at amino acids 1933 and 2163
TPTPTGTQ TPTTTPITTT TTVTPTPTPT GTQTPTTTPI TTT
Database link: Q02817
- Normal colon
We have removed mouse from our list of reactive species based on contradictory results from researchers. We have had increased reports of negative staining results in IHC-P with mouse tissue. We do not batch test this antibody with mouse and we will no longer guarantee it for mouse reactivity.
Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesPurified IgG prepared by affinity chromatography from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab11197 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 10µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. This product does not require protein digestion or antigen retrieval using heat treatment prior to staining of paraffin sections.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 621.5 kDa.|
FunctionCoats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Tissue specificityColon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
Cellular localizationSecreted. In the intestine, secreted into the inner and outer mucus layers.
- Information by UniProt
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
ab11197 has been referenced in 22 publications.
- Tan HY et al. A multi-chamber microfluidic intestinal barrier model using Caco-2 cells for drug transport studies. PLoS One 13:e0197101 (2018). PubMed: 29746551
- Wang YC et al. Notch Signaling Pathway Is Inhibited in the Development of Barrett's Esophagus: An In Vivo and In Vitro Study. Can J Gastroenterol Hepatol 2018:4149317 (2018). PubMed: 29785394
- Hu J et al. Lactobacillus frumenti Facilitates Intestinal Epithelial Barrier Function Maintenance in Early-Weaned Piglets. Front Microbiol 9:897 (2018). PubMed: 29867808
- Song H et al. Worm expulsion of Gymnophalloides seoi from C57BL/6 mice: role of metacercarial exosomes in upregulating TLR2 and MUC2 expression in intestinal tissues. Parasitol Res 117:3309-3314 (2018). PubMed: 30019213
- Bücker R et al. Establishment of a Mucin Secreting Cell Line Cx-03 from an Uterine Carcino Sarcoma. Pharm Res 36:7 (2018). PubMed: 30411161