Overview

  • Product name
  • Description
    Rabbit polyclonal to MUC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic MUC2 peptide (Human) 23 amino acids long.

  • Positive control
    • Human colon carcinoma; LS 174 T colon cancer cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab76774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/50 - 1/100.
ICC Use at an assay dependent concentration.

Target

  • Function
    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity
    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications
    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization
    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • ab76774 at 1/100 dilution, staining MUC2 in human colon cancer tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). The DAB staining procedure was used with hematoxylin as counterstain.

References

This product has been referenced in:
  • Groves HT  et al. Respiratory Disease following Viral Lung Infection Alters the Murine Gut Microbiota. Front Immunol 9:182 (2018). Read more (PubMed: 29483910) »
  • Zhou H  et al. Activation of FXR promotes intestinal metaplasia of gastric cells via SHP-dependent upregulation of the expression of CDX2. Oncol Lett 15:7617-7624 (2018). Read more (PubMed: 29849798) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (Epidermis)
Permeabilization
No
Specification
Epidermis
Blocking step
Dako serum free blocking agent as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
none used
Username

Miss. Crystal Jones

Verified customer

Submitted Feb 19 2018

Thank you for contacting us. Your credit note ID is. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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