Overview

  • Product name

  • Description

    Rabbit polyclonal to MUC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human MUC2 aa 350-450 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q02817
    (Peptide available as ab103274)

  • Positive control

    • This antibody gave a positive signal in human colon tissue sections. ICC/IF: HT29 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab90007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications

    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • IHC image of MUC2 staining in human normal colon FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
  • ab90007 stained in HT29 cells. Cells were fixed with 100% methanol (5 min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% Triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90007 at 5µg/ml and ab7291 (Mouse monoclonal to alpha Tubulin - Loading Control) used at a 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed, (pseudo-colored green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594) preadsorbed, (colored red), both used at a 1/1000 dilution for 1 hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43 µM for 1hour at room temperature.

References

This product has been referenced in:

  • Shen C  et al. Deoxycholic acid (DCA) confers an intestinal phenotype on esophageal squamous epithelium via induction of the stemness-associated reprogramming factors OCT4 and SOX2. Cell Cycle 15:1439-49 (2016). Read more (PubMed: 27096226) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (small intestine-duodenum)
Permeabilization
No
Specification
small intestine-duodenum
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. Eric Wong

Verified customer

Submitted Sep 11 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 19 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Sample
Human Tissue sections (human colon)
Specification
human colon
Permeabilization
No
Fixative
Acetone

Abcam user community

Verified customer

Submitted Dec 01 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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