Anti-MUC2 antibody [B306.1] (ab7848)

Mouse monoclonal MUC2 antibody [B306.1]. Validated in WB, IHC and tested in Human. Cited in 1 publication(s). Independently reviewed in 1 review(s).

Reviews (1)Q&A (1)References (1)

Overview

  • Product name

    Anti-MUC2 antibody [B306.1]
    See all MUC2 primary antibodies

  • Description

    Mouse monoclonal [B306.1] to MUC2

  • Host species

    Mouse

  • Specificity

    This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia.

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic MUC2 tandem repeat consensus sequence.

  • Positive control

    • Colon carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab7848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.

  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.

  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.

  • Post-translational
    modifications

    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.

  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt

  • Database links

    • Alternative names

      • Intestinal mucin 2 antibody
      • Intestinal mucin-2 antibody
      • MLP antibody
      • MUC 2 antibody
      • MUC-2 antibody
      • Muc2 antibody
      • MUC2_HUMAN antibody
      • Mucin 2 antibody
      • Mucin 2 intestinal antibody
      • Mucin 2 intestinal/tracheal antibody
      • Mucin 2 oligomeric mucus/gel forming antibody
      • Mucin 2 precursor antibody
      • Mucin 2 tracheal antibody
      • Mucin like protein antibody
      • Mucin-2 antibody
      • Mucin2 antibody
      • SMUC antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)
      Ab7848 staining human normal colon. Staining is localised to the membrane.
      Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

    References

    This product has been referenced in:

    • Fishbein TM  et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). IHC-Fr ; Human . Read more (PubMed: 19519821) »
    See 1 Publication for this product

    Publishing research using ab7848? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-MUC2 antibody [B306.1]

     Poor
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (GI)
    Loading amount
    50 µg
    Specification
    GI
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    proprietary as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 27 2007

    Question

    I have inquiries about your products, ab7848 and ab8605. A researcher is looking for MUC1 antibody and MUC2 antibody. Please let me know if the ab7848 should detect MUC2 in gastric mucosa and the ab8605 should detect MUC1 in intestinal mucosa, respectively. I can't find these descriptions in your datasheets.

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    Answer

    Thank you for contacting us with your enquiry. The SwissProt database links on the datasheets for these two antibodies give some information on the tissue expression of Muc1 and muc2. Muc 2 (ab7848) is expressed in Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. Muc 1 (ab8605) is expressed on ductal epithelial cells and on activated T-cells. Aberrantly glycosylated forms expressed in human epithelial tumors, such as breast or ovarian cancer and also in non-epithelial tumor cells. I have also searched a reference which gives details of Muc 1 (and Muc 2)expression in normal and tumour gastric and intestinal tissue: J Clin Pathol. 1996 July; 49(7): 560–564. Significance of MUC1 and MUC2 mucin expression in colorectal cancer. Y Ajioka et al. I hope this information is helpful. Should the customer have any further questions, please do not hesitate to contact us again.

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