Anti-MUC2 antibody [B306.1] (ab7848)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)](https://www.abcam.com/ps/products/7/ab7848/Images/ab7848-13302-anti-muc2-antibody-b3061-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)")
Key features and details
- Mouse monoclonal [B306.1] to MUC2
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MUC2 antibody [B306.1]
See all MUC2 primary antibodies -
Description
Mouse monoclonal [B306.1] to MUC2 -
Host species
Mouse -
Specificity
This antibody reacts with MUC2 gene product, a secretory apomucin found in a variety of normal and neoplastic human glandular epithelia. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to MUC2. MUC2 tandem repeat consensus sequence.
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Positive control
- Colon carcinoma
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
B306.1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/4000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
Tissue specificity
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
Post-translational
modificationsO-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
Cellular localization
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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Database links
- Entrez Gene: 4583 Human
- Omim: 158370 Human
- SwissProt: Q02817 Human
- Unigene: 315 Human
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Alternative names
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MUC2 antibody [B306.1] (ab7848)Ab7848 staining human normal colon. Staining is localised to the membrane.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab7848 has been referenced in 1 publication.
- Fishbein TM et al. Rejection reversibly alters enteroendocrine cell renewal in the transplanted small intestine. Am J Transplant 9:1620-8 (2009). IHC-Fr ; Human . PubMed: 19519821