Recombinant Anti-MUC2 antibody [EPR6144] (ab133555)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6144] to MUC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MUC2 antibody [EPR6144]
See all MUC2 primary antibodies -
Description
Rabbit monoclonal [EPR6144] to MUC2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MUC2 aa 350-450. The exact sequence is proprietary.
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Positive control
- Human small intestine lysate; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6144 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 540 kDa (predicted molecular weight: 540 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 540 kDa (predicted molecular weight: 540 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
Tissue specificity
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
Post-translational
modificationsO-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
Cellular localization
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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Database links
- Entrez Gene: 4583 Human
- Omim: 158370 Human
- SwissProt: Q02817 Human
- Unigene: 315 Human
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Alternative names
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
Images
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Anti-MUC2 antibody [EPR6144] (ab133555) at 1/1000 dilution + Human small intestine lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 540 kDa -
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with ab133555 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab133555 has been referenced in 2 publications.
- Wang N et al. HDAC6/HNF4a loop mediated by miR-1 promotes bile acids-induced gastric intestinal metaplasia. Gastric Cancer 24:103-116 (2021). PubMed: 32705446
- Zhang L et al. Mitotic checkpoint kinase Mps1/TTK predicts prognosis of colon cancer patients and regulates tumor proliferation and differentiation via PKCa/ERK1/2 and PI3K/Akt pathway. Med Oncol 37:5 (2019). PubMed: 31720873