Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6144] to MUC2
- Suitable for: WB, IHC-P
- Reacts with: Human
Product nameAnti-MUC2 antibody [EPR6144]
See all MUC2 primary antibodies
DescriptionRabbit monoclonal [EPR6144] to MUC2
Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Human
Synthetic peptide within Human MUC2 aa 350-450. The exact sequence is proprietary.
- Human small intestine lysate; Human colon tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 540 kDa (predicted molecular weight: 540 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCoats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
Tissue specificityColon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
Cellular localizationSecreted. In the intestine, secreted into the inner and outer mucus layers.
- Information by UniProt
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
Anti-MUC2 antibody [EPR6144] (ab133555) at 1/1000 dilution + Human small intestine lysate at 10 µg
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 540 kDa
Observed band size: 540 kDa
Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with ab133555 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab133555 has not yet been referenced specifically in any publications.