Overview

  • Product name

    Anti-MUC2 antibody [EPR6144]
    See all MUC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6144] to MUC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MUC2 aa 350-450. The exact sequence is proprietary.

  • Positive control

    • Human small intestine lysate; Human colon tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 540 kDa (predicted molecular weight: 540 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
    • Tissue specificity

      Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 1 TIL (trypsin inhibitory-like) domain.
      Contains 2 VWFC domains.
      Contains 4 VWFD domains.
    • Post-translational
      modifications

      O-glycosylated.
      May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
      At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
    • Cellular localization

      Secreted. In the intestine, secreted into the inner and outer mucus layers.
    • Information by UniProt
    • Database links

    • Alternative names

      • Intestinal mucin 2 antibody
      • Intestinal mucin-2 antibody
      • MLP antibody
      • MUC 2 antibody
      • MUC-2 antibody
      • Muc2 antibody
      • MUC2_HUMAN antibody
      • Mucin 2 antibody
      • Mucin 2 intestinal antibody
      • Mucin 2 intestinal/tracheal antibody
      • Mucin 2 oligomeric mucus/gel forming antibody
      • Mucin 2 precursor antibody
      • Mucin 2 tracheal antibody
      • Mucin like protein antibody
      • Mucin-2 antibody
      • Mucin2 antibody
      • SMUC antibody
      see all

    Images

    • Anti-MUC2 antibody [EPR6144] (ab133555) at 1/1000 dilution + Human small intestine lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 540 kDa
      Observed band size: 540 kDa

    • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with ab133555 at a dilution of 1/100.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab133555 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133555.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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