Recombinant Anti-MUC2 antibody [EPR6145] (ab134119)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6145] to MUC2
- Suitable for: Indirect ELISA, WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MUC2 antibody [EPR6145]
See all MUC2 primary antibodies -
Description
Rabbit monoclonal [EPR6145] to MUC2 -
Host species
Rabbit -
Tested applications
Suitable for: Indirect ELISA, WB, IHC-Pmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.30 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6145 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Indirect ELISA |
Use a concentration of 1 µg/ml.
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WB |
1/1000 - 1/10000. Detects a band of approximately 110, 540 kDa (predicted molecular weight: 540 kDa).
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IHC-P |
1/15000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Or perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). See IHC antigen retrieval protocols. For unpurified use at 1/250-1/500. |
Notes |
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Indirect ELISA
Use a concentration of 1 µg/ml. |
WB
1/1000 - 1/10000. Detects a band of approximately 110, 540 kDa (predicted molecular weight: 540 kDa). |
IHC-P
1/15000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). See IHC antigen retrieval protocols. For unpurified use at 1/250-1/500. |
Target
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Function
Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer. -
Tissue specificity
Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 1 TIL (trypsin inhibitory-like) domain.
Contains 2 VWFC domains.
Contains 4 VWFD domains. -
Post-translational
modificationsO-glycosylated.
May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway. -
Cellular localization
Secreted. In the intestine, secreted into the inner and outer mucus layers. - Information by UniProt
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Database links
- Entrez Gene: 4583 Human
- Omim: 158370 Human
- SwissProt: Q02817 Human
- Unigene: 315 Human
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Alternative names
- Intestinal mucin 2 antibody
- Intestinal mucin-2 antibody
- MLP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-MUC2 antibody [EPR6145] (ab134119) at 1/2000 dilution ((unpurified))
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysate
Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
Lane 5 : Human small intestine tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 540 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe 110kda band is the C-terminal cleaved fragment, formed by an autocatalytic mechanism triggered by low pH. (PMID: 12582180).
Blocking/Dilution buffer: 5% NFDM/TBST.
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Indirect ELISA using ab134119 at varying antibody concentrations (1000-0 ng/mL) and MUC2 antigen at 1000 ng/mL. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as a secondary antibody.
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Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with unpurified ab134119 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Anti-MUC2 antibody [EPR6145] (ab134119) at 1/10000 dilution (Purified) + Human colon lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 540 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?MUC2 could be cleaved into three fragments with the molecular weight 250, 130 and 110 KD as is described in PMID: 12582180.
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Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using unpurified ab134119 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab134119 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human brain tissue using unpurified ab134119 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab134119 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using unpurified ab134119 showing -ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab134119 has been referenced in 17 publications.
- Alula KM et al. Inner mitochondrial membrane protein Prohibitin 1 mediates Nix-induced, Parkin-independent mitophagy. Sci Rep 13:18 (2023). PubMed: 36593241
- Zhang L et al. HDAC6/FOXP3/HNF4α axis promotes bile acids induced gastric intestinal metaplasia. Am J Cancer Res 12:1409-1422 (2022). PubMed: 35411233
- Qian Y et al. Atypical Mucin Expression Predicts Worse Overall Survival in Resectable Pancreatic Ductal Adenocarcinoma. J Immunol Res 2022:7353572 (2022). PubMed: 35910854
- Wang H et al. Low crude protein diets supplemented with casein hydrolysate enhance the intestinal barrier function and decrease the pro-inflammatory cytokine expression in the small intestine of pigs. Anim Nutr 7:770-778 (2021). PubMed: 34466681
- Van Acker SI et al. Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures. Int J Mol Sci 22:N/A (2021). PubMed: 34638869