Overview

  • Product name

    Anti-MUC2 antibody [EPR6145]
    See all MUC2 primary antibodies
  • Description

    Rabbit monoclonal [EPR6145] to MUC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human MUC2.

  • Positive control

    • WB: Caco2, SKBR3, HT1376, SH SY5Y and SK OV3 cell lysates and human small intestine and colon lysates. IHC-P: Human colon tissue.
  • General notes

    Mouse and rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110, 540 kDa (predicted molecular weight: 540 kDa).
IHC-P 1/15000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

for unpurified use at 1/250-1/500

Target

  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications

    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling MUC2 with purified ab134119 at 1/15,000 dilution (0.01 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • All lanes : Anti-MUC2 antibody [EPR6145] (ab134119) at 1/2000 dilution ((unpurified))

    Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 3 : HT-1376 (Human urinary bladder carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 4 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate with 5% NFDM/TBST
    Lane 5 : Human small intestine tissue lysate with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 540 kDa
    Observed band size: 110 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    The 110kda band is the C-terminal cleaved fragment, formed by an autocatalytic mechanism triggered by low pH. (PMID: 12582180)

  • Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with unpurified ab134119 at 1/250.

  • Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using unpurified ab134119 showing +ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using unpurified ab134119 showing -ve staining.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Immunohistochemical analysis of paraffin embedded normal Human brain tissue using unpurified ab134119 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab134119 showing -ve staining.

  • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using unpurified ab134119 showing -ve staining.

References

This product has been referenced in:

  • Wei J  et al. Establishment and characterization of the GC-030-35 cell line derived from gastric hepatoid adenocarcinoma. Cancer Manag Res 11:1275-1287 (2019). Read more (PubMed: 30799954) »
  • Tang X  et al. Internal biliary drainage superior to external biliary drainage in improving gut mucosa barrier because of goblet cells and mucin-2 up-regulation. Biosci Rep 38:N/A (2018). Read more (PubMed: 29599128) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
ELISA
Sample
Human Purified protein (mucin extracted from feces samples)
Specification
mucin extracted from feces samples
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Type
Indirect

Abcam user community

Verified customer

Submitted Sep 19 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up