Recombinant
RabMAb

Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free (ab177278)

Overview

  • Product name
    Anti-MUC2 antibody [EPR6145] - Low endotoxin, Azide free
    See all MUC2 primary antibodies
  • Description
    Rabbit monoclonal [EPR6145] to MUC2 - Low endotoxin, Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • General notes

    ab177278 is a PBS-only buffer version of ab134119, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab134119 for information on protocols, dilutions, and image data.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab177278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 110, 540 kDa (predicted molecular weight: 540 kDa).
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity
    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications
    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization
    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links
  • Alternative names
    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • This IHC data was generated using the same anti-MUC2 antibody clone, EPR6145, in a different buffer formulation (cat# ab134119).

    Immunohistochemical analysis of paraffin embedded Human colon tissue labelling MUC2 with ab134119 at 1/250.

  • This IHC data was generated using the same anti-MUC2 antibody clone, EPR6145, in a different buffer formulation (cat# ab134119).

    Immunohistochemical analysis of paraffin embedded Human Colonic adenocarcinoma tissue using ab134119 showing +ve staining.

References

ab177278 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab177278.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up