Overview

  • Product name

    Anti-MUC2 antibody [SPM512]
    See all MUC2 primary antibodies
  • Description

    Mouse monoclonal [SPM512] to MUC2
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MUC2.
    Database link: Q02817

  • Positive control

    • IHC-P: Human small intestine, colon, colon adenocarcinoma, prostate adenocarcinoma and stomach adenocarcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab231427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.
  • Tissue specificity

    Colon, small intestine, colonic tumors, bronchus, cervix and gall bladder.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 TIL (trypsin inhibitory-like) domain.
    Contains 2 VWFC domains.
    Contains 4 VWFD domains.
  • Post-translational
    modifications

    O-glycosylated.
    May undergo proteolytic cleavage in the outer mucus layer of the colon, contributing to the expanded volume and loose nature of this layer which allows for bacterial colonization in contrast to the inner mucus layer which is dense and devoid of bacteria.
    At low pH of 6 and under, undergoes autocatalytic cleavage in vitro in the N-terminal region of the fourth VWD domain. It is likely that this also occurs in vivo and is triggered by the low pH of the late secretory pathway.
  • Cellular localization

    Secreted. In the intestine, secreted into the inner and outer mucus layers.
  • Information by UniProt
  • Database links

  • Alternative names

    • Intestinal mucin 2 antibody
    • Intestinal mucin-2 antibody
    • MLP antibody
    • MUC 2 antibody
    • MUC-2 antibody
    • Muc2 antibody
    • MUC2_HUMAN antibody
    • Mucin 2 antibody
    • Mucin 2 intestinal antibody
    • Mucin 2 intestinal/tracheal antibody
    • Mucin 2 oligomeric mucus/gel forming antibody
    • Mucin 2 precursor antibody
    • Mucin 2 tracheal antibody
    • Mucin like protein antibody
    • Mucin-2 antibody
    • Mucin2 antibody
    • SMUC antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for MUC2 using ab231427 at 1/200 dilution in immunohistochemical analysis.

References

ab231427 has not yet been referenced specifically in any publications.

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