Key features and details
- Rabbit polyclonal to MUC20
- Suitable for: IHC-P, WB
- Reacts with: Rat
- Isotype: IgG
Product nameAnti-MUC20 antibody
See all MUC20 primary antibodies
DescriptionRabbit polyclonal to MUC20
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat
Recombinant fragment corresponding to Rat MUC20 aa 113-347. (N-terminal His-tag and GST-tag; Expressed in E.coli).
TTPAKDSVAFSISHIKLTTCITEIETTITISGTPDASRSPAEAMTALSAS EMLTLPPSTEAKPVVPKTTSSSGILSTATTLALATTLEGTLPASGITESE IAVAQTPTSSGTSATGGTQVTVRRNPLEDTSALSSETQSHTEVFGTITVP TVAGSTVGEATSLVSSTALDSSLSAVVTTKGSASSETLTTDNTTNSSFLT GSRPPSLIYSTTASTSERTNVTLTKTTASPKTPMN
Database link: B2RZ35
- IHC-P: Rat liver, testis and kidney tissues. WB: Rat serum; Rat liver and kidney lysates; Recombinant rat MUC20 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab231659 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 43 kDa.|
FunctionMay regulate MET signaling cascade. Seems to decrease hepatocyte growth factor (HGF)-induced transient MAPK activation. Blocks GRB2 recruitment to MET thus suppressing the GRB2-RAS pathway. Inhibits HGF-induced proliferation of MMP1 and MMP9 expression.
Tissue specificityHighly expressed in kidney, moderately in placenta, lung, prostate, liver, and digestive system. In the kidney, localized in the proximal tubules but not in the glomerulus or distal tubules. Detected in most of the male urogenital tract epithelia, with the exception of epididymis.
Cellular localizationSecreted. Apical cell membrane. Basolateral cell membrane. Cell projection > microvillus membrane.
- Information by UniProt
- MUC 20 antibody
- MUC-20 antibody
- Muc20 antibody
Paraffin-embedded rat liver tissue stained for MUC20 using ab231659 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded rat testis tissue stained for MUC20 using ab231659 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded rat kidney tissue stained for MUC20 using ab231659 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-MUC20 antibody (ab231659) at 1 µg/ml + Recombinant rat MUC20 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
All lanes : Anti-MUC20 antibody (ab231659) at 1 µg/ml
Lane 1 : Rat serum
Lane 2 : Rat liver lysate
Lane 3 : Rat kidney lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 43 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231659 has not yet been referenced specifically in any publications.