Key features and details
- Mouse monoclonal [3C7.D8.E11.F5] to MUC4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MUC4 antibody [3C7.D8.E11.F5]
See all MUC4 primary antibodies
DescriptionMouse monoclonal [3C7.D8.E11.F5] to MUC4
Antibody shows no cross reactivity to non-mucilated proteins.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MUC4 (internal sequence).
Database link: Q99102
- WB: HPAC, HPAF-II, and Capan2 cell lysate.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Potassium phosphate
Concentration information loading...
PurityProtein A purified
Purification notesPurified from TCS.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab263957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 232 kDa.
1/1000. Predicted molecular weight: 232 kDa.
FunctionMay play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation.
Tissue specificityExpressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines.
Sequence similaritiesContains 1 AMOP domain.
Contains 2 EGF-like domains.
Contains 1 NIDO domain.
Contains 1 VWFD domain.
Developmental stageExpressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation.
modificationsProteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
mucrnin-4 alpha chain is highly O-glycosylated.
mucin-4 beta chain is predominantly N-glycosylated.
Cellular localizationSecreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing.
- Information by UniProt
- Ascites sialoglycoprotein 1 antibody
- Ascites sialoglycoprotein 2 antibody
- Ascites sialoglycoprotein antibody
All lanes : Anti-MUC4 antibody [3C7.D8.E11.F5] (ab263957) at 1/1000 dilution (in 2% BSA for 2 hrs at RT)
Lane 1 : HPAF-II cell lysate
Lane 2 : HPAC cell lysate
Lane 3 : Capan2 cell lysate
Lane 4 : MiaPaCa2 cell lysate
Lane 5 : Panc-1 cell lysate
All lanes : Goat Anti-Mouse IgG HRP in 5% BSA/PBS/0.1% Tween-20 for 1 hr at RT at 1/70000 dilution
Predicted band size: 232 kDa
Highly glycosylated protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab263957 has not yet been referenced specifically in any publications.