Anti-MUC4 antibody [5B12] (ab60720)
Key features and details
- Mouse monoclonal [5B12] to MUC4
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-MUC4 antibody [5B12]
See all MUC4 primary antibodies -
Description
Mouse monoclonal [5B12] to MUC4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment with tag: GVSLFPYGAD AGDLEFVRRT VDFTSPLFKP ATGFPLGSSL RDSLYFTDNG QIIFPESDYQ IFSYPNPLPT GFTGRDPVAL VAPFWDDADF STGRGTTFYQ EYETFYGEHS , corresponding to amino acids 79-189 of Human MUC4
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Positive control
- WB: HeLa cell lysate. IHC-P: Human stomach tissue. Flow Cyt: A549 cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 12 Feb 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
5B12 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 235 kDa).
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IHC-P | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 235 kDa). |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
May play a role in tumor progression. Ability to promote tumor growth may be mainly due to repression of apoptosis as opposed to proliferation. Has anti-adhesive properties. Seems to alter cellular behavior through both anti-adhesive effects on cell-cell and cell-extracellular matrix interactions and in its ability to act as an intramembrane ligand for ERBB2. Plays an important role in cell proliferation and differentiation of epithelial cells by inducing specific phosphorylation of ERBB2. The MUC4-ERBB2 complex causes site-specific phosphorylation of the ERBB2 'Tyr-1248'. In polarized epithelilal cells segragates ERBB2 and other ERBB receptors and prevents ERBB2 from acting as a coreceptor. The interaction with ERBB2 leads to enhanced expression of CDKN1B. The formation of a MUC4-ERBB2-ERBB3-NRG1 complex leads to down-regulation of CDKN1B, resulting in repression of apoptosis and stimulation of proliferation. -
Tissue specificity
Expressed in the thymus, thyroid, lung, trachea, esophagus, stomach, small intestine, colon, testis, prostate, ovary, uterus, placenta, and mammary and salivary glands. Expressed in carcinomas arising from some of these epithelia, such as lung cancers, squamous cell carcinomas of the upper aerodigestive tract, mammary carcinomas, biliary tract, colon, and cervix cancers. Minimally or not expressed in the normal pancreas or chronic pancreatitis, but is highly expressed in pancreatic tumors and pancreatic tumor cell lines. -
Sequence similarities
Contains 1 AMOP domain.
Contains 2 EGF-like domains.
Contains 1 NIDO domain.
Contains 1 VWFD domain. -
Developmental stage
Expressed early in the primitive gut before respiratory and digestive epithelial cells have acquired their tissue and cell specificity. Expressed at the basal surface of the epithelium from week 14 to 26 weeks and then predominantly localized in only parietal cells. Immediately before birth, found in the cytoplasm of the mucous columnar epithelial cells. In the embryo expressed in skin, then disappears late in gestation. -
Post-translational
modificationsProteolytically cleaved into 2 chains, mucin-4 alpha chain and mucin-4 beta chain.
mucrnin-4 alpha chain is highly O-glycosylated.
mucin-4 beta chain is predominantly N-glycosylated. -
Cellular localization
Secreted; Cell membrane and Membrane. Secreted. Isoforms lacking the Cys-rich region, EGF-like domains and transmembrane region are secreted. Secretion occurs by splicing or proteolytic processing. - Information by UniProt
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Database links
- Entrez Gene: 4585 Human
- Omim: 158372 Human
- SwissProt: Q99102 Human
- Unigene: 369646 Human
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Alternative names
- Ascites sialoglycoprotein 1 antibody
- Ascites sialoglycoprotein 2 antibody
- Ascites sialoglycoprotein antibody
see all
Images
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Anti-MUC4 antibody [5B12] (ab60720) at 1 µg/ml + HeLa cell lysate at 25 µg
Predicted band size: 235 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Additional bands at: 52 kDa, 65 kDa. We are unsure as to the identity of these extra bands.This image was generated using the ascites version of the product.
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MUC4 antibody (ab60720) used in immunohistochemistry at 3ug/mL on formalin fixed and paraffin embedded human stomach.
This image was generated using the ascites version of the product.
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Overlay histogram showing A549 cells stained with ab60720 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60720, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
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Datasheet download
References (22)
ab60720 has been referenced in 22 publications.
- Lungova V et al. Exposure to e-cigarette vapor extract induces vocal fold epithelial injury and triggers intense mucosal remodeling. Dis Model Mech 15:N/A (2022). PubMed: 35770504
- He N et al. Nano pom-poms prepared exosomes enable highly specific cancer biomarker detection. Commun Biol 5:660 (2022). PubMed: 35787656
- Collin J et al. Co-expression of SARS-CoV-2 entry genes in the superficial adult human conjunctival, limbal and corneal epithelium suggests an additional route of entry via the ocular surface. Ocul Surf 19:190-200 (2021). PubMed: 32502616
- Cotoraci CA et al. The morphological changes of the colonic goblet cells and mucin profile in oncohematological patients under Epirubicin-based chemotherapy. Rom J Morphol Embryol 61:1121-1128 (2020). PubMed: 34171061
- Genschmer KR et al. Activated PMN Exosomes: Pathogenic Entities Causing Matrix Destruction and Disease in the Lung. Cell 176:113-126.e15 (2019). PubMed: 30633902