Anti-MUC5B antibody [19.4E] (ab77995)
Key features and details
- Mouse monoclonal [19.4E] to MUC5B
- Suitable for: ELISA, IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG1
Overview
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Product name
Anti-MUC5B antibody [19.4E]
See all MUC5B primary antibodies -
Description
Mouse monoclonal [19.4E] to MUC5B -
Host species
Mouse -
Specificity
Does not react with human Mucin 5A or 5C.
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Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Monkey -
Immunogen
Synthetic peptide corresponding to Human MUC5B (N terminal). Un-glycosylated, serine and threonine free synthetic peptide from the N terminal region of human MUC5B.
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General notes
This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
19.4E -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
Deglycosylating the tissue is critical to expose epitope. |
IHC-Fr | (1) |
Use at an assay dependent concentration.
|
WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Deglycosylating the tissue is critical to expose epitope. |
IHC-Fr
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus. -
Tissue specificity
Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 3 HAT repeats.
Contains 3 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding. -
Post-translational
modificationsHighly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 727897 Human
- Entrez Gene: 74180 Mouse
- Omim: 600770 Human
- SwissProt: Q9HC84 Human
- SwissProt: Q8K1G6 Mouse
- Unigene: 523395 Human
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Alternative names
- 2300002I04Rik antibody
- A130042M24 antibody
- AV085033 antibody
see all
Images
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ab77995 staining MUC5B in murine asthma lung tissue by Immunohistochemistry (Frozen sections). Tissue was fixed with formaldehyde, followed by incubation with the primary antibody at 4µg/ml for 16 hours at 4°C. ab77995 was coupled to biotin and Streptavidin 488 (1/250) was used to amplify the signal. Counterstained with DAPI (blue).
This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab77995 has been referenced in 23 publications.
- Wang Z et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
- Huang X et al. MUC5B regulates goblet cell differentiation and reduces inflammation in a murine COPD model. Respir Res 23:11 (2022). PubMed: 35042537
- Gurumurthy RK et al. Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection. Nat Protoc 17:1658-1690 (2022). PubMed: 35546639
- Ciccimarra R et al. The normal and fibrotic mouse lung classified by spatial proteomic analysis. Sci Rep 12:8742 (2022). PubMed: 35610327
- Devadoss D et al. Immunomodulatory LncRNA on antisense strand of ICAM-1 augments SARS-CoV-2 infection-associated airway mucoinflammatory phenotype. iScience 25:104685 (2022). PubMed: 35789750