Overview

  • Product name

    Anti-MUC5B antibody [6F10-E4]
    See all MUC5B primary antibodies
  • Description

    Mouse monoclonal [6F10-E4] to MUC5B
  • Host species

    Mouse
  • Specificity

    Non glycosylated domain of mucin MUC5B.
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide

    RNREQVGKF

    from 4 of the cysteine domains of the tandem repeat region of Human MUC5B conjugated to KLH.

  • General notes


    Detection of respiratory and salivary mucins in clinical and experimental material.

Properties

Applications

Our Abpromise guarantee covers the use of ab105460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 596 kDa. PubMed: 14678646

Please consult PubMed ID 14678646 for the recommended electrophoresis conditions.

Target

  • Function

    Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
  • Tissue specificity

    Expressed on surface airway epithelia. Expressed mainly in mucous cells of submucosal glands of airway tissues. Highly expressed in the sublingual gland. Also found in submaxillary glands, endocervix, gall bladder, and pancreas.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 3 HAT repeats.
    Contains 3 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • Domain

    The cysteine residues in the Cys-rich subdomain repeats are not involved in disulfide bonding.
  • Post-translational
    modifications

    Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2300002I04Rik antibody
    • A130042M24 antibody
    • AV085033 antibody
    • Cervical mucin antibody
    • Cervical mucin MUC5B antibody
    • High molecular weight salivary mucin MG1 antibody
    • MG1 antibody
    • MUC-5B antibody
    • MUC5 antibody
    • MUC5B antibody
    • MUC5B_HUMAN antibody
    • MUC9 antibody
    • Mucin 5 subtype B, tracheobronchial antibody
    • Mucin 5B antibody
    • Mucin 5B oligomeric mucus/gel forming antibody
    • Mucin-5 subtype B antibody
    • Mucin-5B antibody
    • Sublingual gland mucin antibody
    • tracheobronchial antibody
    see all

References

This product has been referenced in:

  • van Leeuwen S  et al. Early salivary changes in multiple myeloma patients undergoing autologous HSCT. Oral Dis 24:972-982 (2018). Read more (PubMed: 29637662) »
  • Lachowicz-Scroggins ME  et al. Abnormalities in MUC5AC and MUC5B Protein in Airway Mucus in Asthma. Am J Respir Crit Care Med 194:1296-1299 (2016). Read more (PubMed: 27845589) »
See all 3 Publications for this product

Customer reviews and Q&As

Question
Answer

Thank you for contacting us.

I have been searching the antibodies as per your requirements. We have different anti Mucin 5AC antibodies however I am not sure which would better suits you because is not very clear from the immunogen.

Regarding the anti MUC 5B we have ab105460 which detects the non glycosylated domain of MUC5B. Please check he datasheet.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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