Overview

  • Product name

    Anti-MUM1 antibody [EP5699]
    See all MUM1 primary antibodies
  • Description

    Rabbit monoclonal [EP5699] to MUM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IP, Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human MUM1 (UniProt ID: Q15306).

  • Positive control

    • Namalwa, HuT-78, and IM-9 cell lysates, Human lymphoma and Human tonsil tissues
  • General notes

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 52 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP 1/100 - 1/500.
Flow Cyt 1/100 - 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/250 - 1/500.

Target

  • Function

    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
  • Tissue specificity

    Lymphoid cells.
  • Involvement in disease

    Defects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon regulatory factor 4 antibody
    • IRF 4 antibody
    • IRF-4 antibody
    • Irf4 antibody
    • IRF4_HUMAN antibody
    • LSIRF antibody
    • Lymphocyte specific interferon regulatory factor antibody
    • Lymphocyte specific IRF antibody
    • Lymphocyte-specific interferon regulatory factor antibody
    • Multiple myeloma oncogene 1 antibody
    • MUM 1 antibody
    • MUM1 antibody
    • NF EM5 antibody
    • NF-EM5 antibody
    • NFEM5 antibody
    • PU.1 interaction partner antibody
    • Sfpi1/PU.1 interaction partner antibody
    • Transcriptional activator PIP antibody
    see all

Images

  • All lanes : Anti-MUM1 antibody [EP5699] (ab133590) at 1/10000 dilution

    Lane 1 : Namalwa cell lysate
    Lane 2 : HuT78 cell lysate
    Lane 3 : IM9 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 52 kDa

  • Overlay histogram showing Ramos cells stained with ab133590 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133590, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Ramos cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling MUM1, using ab133590 at a dilution of 1/250.
  • Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling MUM1, using ab133590 at a dilution of 1/250.

References

This product has been referenced in:

  • Behrens NE  et al. Serum Amyloid P Component Binds Fungal Surface Amyloid and Decreases Human Macrophage Phagocytosis and Secretion of Inflammatory Cytokines. MBio 10:N/A (2019). Read more (PubMed: 30862745) »
  • Su CY  et al. High density of CD68+ tumor-associated macrophages predicts a poor prognosis in gastric cancer mediated by IL-6 expression. Oncol Lett 15:6217-6224 (2018). Read more (PubMed: 29616104) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA
Permeabilization
Yes - 3% H2O2
Specification
Lymph node
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 12 2019

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5% · Temperature: 22°C
Sample
Human Cell (U266 and Daudi)
Specification
U266 and Daudi
Permeabilization
Yes - 0.5% Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 09 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up