Overview

  • Product name

    Anti-MUM1 antibody [EP5699] (Biotin)
    See all MUM1 primary antibodies
  • Description

    Rabbit monoclonal [EP5699] to MUM1 (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MUM1 aa 100-450. The exact sequence is proprietary.
    Database link: Q15306

  • Positive control

    • IHC-P: Human lymph node (Hodgkin's lymphoma) tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab201482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
  • Tissue specificity

    Lymphoid cells.
  • Involvement in disease

    Defects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
  • Sequence similarities

    Belongs to the IRF family.
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Interferon regulatory factor 4 antibody
    • IRF 4 antibody
    • IRF-4 antibody
    • Irf4 antibody
    • IRF4_HUMAN antibody
    • LSIRF antibody
    • Lymphocyte specific interferon regulatory factor antibody
    • Lymphocyte specific IRF antibody
    • Lymphocyte-specific interferon regulatory factor antibody
    • Multiple myeloma oncogene 1 antibody
    • MUM 1 antibody
    • MUM1 antibody
    • NF EM5 antibody
    • NF-EM5 antibody
    • NFEM5 antibody
    • PU.1 interaction partner antibody
    • Sfpi1/PU.1 interaction partner antibody
    • Transcriptional activator PIP antibody
    see all

Images

  • IHC image of IRF4 staining in a section of formalin-fixed paraffin-embedded human lymph node (Hodgkin's lymphoma)*, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab201482 at 1/100 dilution for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

References

ab201482 has not yet been referenced specifically in any publications.

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