Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP114] to MUM1 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Product nameAnti-MUM1 antibody [SP114] - C-terminal
See all MUM1 primary antibodies
DescriptionRabbit monoclonal [SP114] to MUM1 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Cow, Dog
Synthetic peptide within Human MUM1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q15306
- IHC-P: Hodgkins lymphoma, HK lymphoma, " Human Hodgkins lymphoma", and Human colon tissue; WB: Ramos, 293T, IM-9 and HuT-78 cell lysate Flow Cyt: Ramos cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A/G purified
Purification notesPurified from TCS by protein A/G.
Our Abpromise guarantee covers the use of ab227646 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Predicted molecular weight: 52 kDa.
Primary antibody incubation for 1 hour at room temperature.
Perform heat mediated antigen retrieval with 10 mM EDTA buffer before commencing with IHC staining protocol.
Primary antibody incubation for 30 minutes at room temperature.
FunctionTranscriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells.
Tissue specificityLymphoid cells.
Involvement in diseaseDefects in IRF4 are a cause of multiple myeloma (MM) [MIM:254500]. MM is a malignant tumor of plasma cells usually arising in the bone marrow and characterized by diffuse involvement of the skeletal system, hyperglobulinemia, Bence-Jones proteinuria and anemia. Complications of multiple myeloma are bone pain, hypercalcemia, renal failure and spinal cord compression. The aberrant antibodies that are produced lead to impaired humoral immunity and patients have a high prevalence of infection. Amyloidosis may develop in some patients. Multiple myeloma is part of a spectrum of diseases ranging from monoclonal gammopathy of unknown significance (MGUS) to plasma cell leukemia. Note=A chromosomal aberration involving IRF4 is found in multiple myeloma. Translocation t(6;14)(p25;q32) with the IgH locus.
Sequence similaritiesBelongs to the IRF family.
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.
- Information by UniProt
- Interferon regulatory factor 4 antibody
- IRF 4 antibody
- IRF-4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
All lanes : Anti-MUM1 antibody [SP114] - C-terminal (ab227646) at 0.25 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate
Lane 2 : HuT-78 (Human Sezary syndrome cutaneous T lymphocyte) cell lysate
Lane 3 : IM-9 (Human peripheral blood lymphoblast) cell lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 52 kDa
Observed band size: 52 kDa
Blocking/Diluting buffer and concentration: 5% NFDM /TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling MUM1 with ab227646 at 1/100 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.
Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling MUM1 with purified ab227646 at 1:230 dilution (1.01 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Anti-MUM1 antibody [SP114] - C-terminal (ab227646) at 1/100 dilution + Ramos (human Burkitt's lymphoma cell line) cell lysate
Predicted band size: 52 kDa
Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for MUM1 using ab227646 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227646 has been referenced in 1 publication.
- Reinke S et al. Round-robin test for the cell-of-origin classification of diffuse large B-cell lymphoma-a feasibility study using full slide staining. Virchows Arch N/A:N/A (2018). PubMed: 29730836