Overview

  • Product name

    Anti-Mumps nucleoprotein antibody [5E3]
    See all Mumps nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [5E3] to Mumps nucleoprotein
  • Host species

    Mouse
  • Tested applications

    Suitable for: Indirect ELISA, WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant mumps NP. Gloucester strain (Glouc 1/UK96)

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5E3
  • Myeloma

    NS0/1
  • Isotype

    IgG2a
  • Light chain type

    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9879 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA
WB
  • Application notes
    Indirect ELISA: Use at 1/1000 to 1/5000
    Western Blot: Use at 1/500 to 1/1000
  • Target

    Images

    • Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

      Western blot of yeast-derived mumps NP using ab9879 at a concentration of 0.5 µg/ml.

    References

    This product has been referenced in:

    • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). Read more (PubMed: 11748668) »
    • A.Þvirblienë  et al. Studies on the structure and antigenic properties of mumps virus nucleocapsid protein produced in yeast cells. Immunol Lett. Abs. of 14th Eur Immunol. meet. EFIS : (2000). Read more (PubMed: ) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Answer

    Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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