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  1. Link

    mumps-nucleoprotein-antibody-7b10-ab9880.pdf

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Microbiology Organism Virus RNA Virus single stranded RNA negative strand virus Mumps
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Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (11)

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Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

    Key features and details

    • Mouse monoclonal [7B10] to Mumps nucleoprotein
    • Suitable for: WB, Indirect ELISA
    • Isotype: IgG2a

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    Overview

    • Product name

      Anti-Mumps nucleoprotein antibody [7B10]
      See all Mumps nucleoprotein primary antibodies
    • Description

      Mouse monoclonal [7B10] to Mumps nucleoprotein
    • Host species

      Mouse
    • Tested applications

      Suitable for: WB, Indirect ELISAmore details
    • Species reactivity

      Reacts with: Other species
    • Immunogen

      Tissue, cells or virus corresponding to Mumps nucleoprotein. Recombinant mumps NP. Gloucester strain (Glouc 1/UK96)

    • General notes

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR293636-4 and GR137414-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.2
      Preservative: 0.1% Sodium azide
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Monoclonal
    • Clone number

      7B10
    • Myeloma

      NS0/1
    • Isotype

      IgG2a
    • Light chain type

      unknown
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • single stranded RNA negative strand virus
      • Mumps

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9880 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
    Indirect ELISA
    1/1000 - 1/10000.
    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
    Indirect ELISA
    1/1000 - 1/10000.

    Target

    • Database links

      • Entrez Gene: 1489766 Other species
      • SwissProt: Q783W2 Other species
      • Alternative names

        • NP antibody
        • Nucleocapsid protein antibody
        • Nucleoprotein antibody

      Images

      • Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)
        Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

        Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
        Lane 1 - prestained standard proteins
        Lane 2 - mumps NP
        Antibody 7B10 at a concentration of 0.5 µg/ml.

        Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
        Lane 1 - prestained standard proteins
        Lane 2 - mumps NP
        Antibody 7B10 at a concentration of 0.5 µg/ml.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (11)

      Publishing research using ab9880? Please let us know so that we can cite the reference in this datasheet.

      ab9880 has been referenced in 11 publications.

      • Chen R  et al. Characterization of an Antiviral Component in Human Seminal Plasma. Front Immunol 12:580454 (2021). PubMed: 33679733
      • Hao X  et al. Development of Improved Mumps Vaccine Candidates by Mutating Viral mRNA Cap Methyltransferase Sites in the Large Polymerase Protein. Virol Sin 36:521-536 (2021). PubMed: 33284397
      • Marques RF  et al. Protective Malaria Vaccine in Mice Based on the Plasmodium vivax Circumsporozoite Protein Fused with the Mumps Nucleocapsid Protein. Vaccines (Basel) 8:N/A (2020). PubMed: 32325874
      • Wu H  et al. Mumps virus infection disrupts blood-testis barrier through the induction of TNF-a in Sertoli cells. FASEB J 33:12528-12540 (2019). PubMed: 31450968
      • Sun AC  et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 05 2007

      Answer

      Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

      Read More

      Abcam Scientific Support

      Answered on Feb 06 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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