Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

Submit a review Q&A (1)References (7)

Key features and details

  • Mouse monoclonal [7B10] to Mumps nucleoprotein
  • Suitable for: WB, Indirect ELISA
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Mumps nucleoprotein antibody [7B10]
    See all Mumps nucleoprotein primary antibodies

  • Description

    Mouse monoclonal [7B10] to Mumps nucleoprotein

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, Indirect ELISAmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Recombinant mumps NP. Gloucester strain (Glouc 1/UK96)

  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR293636-4 and GR137414-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    7B10

  • Myeloma

    NS0/1

  • Isotype

    IgG2a

  • Light chain type

    unknown

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
Indirect ELISA 1/1000 - 1/10000.

Target

Images

  • Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)
    Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

    Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
    Lane 1 - prestained standard proteins
    Lane 2 - mumps NP
    Antibody 7B10 at a concentration of 0.5 µg/ml.

    Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
    Lane 1 - prestained standard proteins
    Lane 2 - mumps NP
    Antibody 7B10 at a concentration of 0.5 µg/ml.

References

Publishing research using ab9880? Please let us know so that we can cite the reference in this datasheet.

ab9880 has been referenced in 7 publications.

  • Sun AC  et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
  • Lo MK  et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). PubMed: 28262699
  • Gouma S  et al. Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection. Vaccine 34:4166-4171 (2016). PubMed: 27372154
  • Han WG  et al. Development of an IFN? ELISPOT for the analysis of the human T cell response against mumps virus. J Immunol Methods 431:52-9 (2016). PubMed: 26872407
  • Stewart CE  et al. Inhibitors of the interferon response enhance virus replication in vitro. PLoS One 9:e112014 (2014). PubMed: 25390891
  • Vaidya SR  et al. Development of a focus reduction neutralization test (FRNT) for detection of mumps virus neutralizing antibodies. J Virol Methods 163:153-6 (2010). ELISA . PubMed: 19761798
  • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). PubMed: 11748668

Customer reviews and Q&As

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Question

Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

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Answer

Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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