Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

Submit a review Q&A (1)References (9)
Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

    Key features and details

    • Mouse monoclonal [7B10] to Mumps nucleoprotein
    • Suitable for: WB, Indirect ELISA
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-Mumps nucleoprotein antibody [7B10]
      See all Mumps nucleoprotein primary antibodies

    • Description

      Mouse monoclonal [7B10] to Mumps nucleoprotein

    • Host species

      Mouse

    • Tested applications

      Suitable for: WB, Indirect ELISAmore details

    • Species reactivity

      Reacts with: Other species

    • Immunogen

      Recombinant mumps NP. Gloucester strain (Glouc 1/UK96)

    • General notes

      This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR293636-4 and GR137414-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7.2
      Preservative: 0.1% Sodium azide
    • Concentration information loading...

    • Purity

      Protein A purified

    • Clonality

      Monoclonal

    • Clone number

      7B10

    • Myeloma

      NS0/1

    • Isotype

      IgG2a

    • Light chain type

      unknown

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab9880 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
    Indirect ELISA
    1/1000 - 1/10000.
    Notes
    WB
    1/1000 - 1/5000. Predicted molecular weight: 66 kDa.
    Indirect ELISA
    1/1000 - 1/10000.

    Target

    Images

    • Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)
      Western blot - Anti-Mumps nucleoprotein antibody [7B10] (ab9880)

      Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
      Lane 1 - prestained standard proteins
      Lane 2 - mumps NP
      Antibody 7B10 at a concentration of 0.5 µg/ml.

      Immunoblot of yeast-derived mumps NP using monoclonal antibody 7B10.
      Lane 1 - prestained standard proteins
      Lane 2 - mumps NP
      Antibody 7B10 at a concentration of 0.5 µg/ml.

    References (9)

    Publishing research using ab9880? Please let us know so that we can cite the reference in this datasheet.

    ab9880 has been referenced in 9 publications.

    • Marques RF  et al. Protective Malaria Vaccine in Mice Based on the Plasmodium vivax Circumsporozoite Protein Fused with the Mumps Nucleocapsid Protein. Vaccines (Basel) 8:N/A (2020). PubMed: 32325874
    • Wu H  et al. Mumps virus infection disrupts blood-testis barrier through the induction of TNF-a in Sertoli cells. FASEB J 33:12528-12540 (2019). PubMed: 31450968
    • Sun AC  et al. High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array. IEEE J Solid-State Circuits 53:2054-2064 (2018). PubMed: 30559530
    • Lo MK  et al. GS-5734 and its parent nucleoside analog inhibit Filo-, Pneumo-, and Paramyxoviruses. Sci Rep 7:43395 (2017). PubMed: 28262699
    • Gouma S  et al. Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection. Vaccine 34:4166-4171 (2016). PubMed: 27372154
    • Han WG  et al. Development of an IFN? ELISPOT for the analysis of the human T cell response against mumps virus. J Immunol Methods 431:52-9 (2016). PubMed: 26872407
    • Stewart CE  et al. Inhibitors of the interferon response enhance virus replication in vitro. PLoS One 9:e112014 (2014). PubMed: 25390891
    • Vaidya SR  et al. Development of a focus reduction neutralization test (FRNT) for detection of mumps virus neutralizing antibodies. J Virol Methods 163:153-6 (2010). ELISA . PubMed: 19761798
    • Samuel D  et al. High level expression of recombinant mumps nucleoprotein in Saccharomyces cerevisiae and its evaluation in mumps IgM serology. J Med Virol 66:123-30 (2002). PubMed: 11748668

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    Please provide details of the stains of mumps virus that Abcam mumps antibodies have been tested on.

    Read More
    Answer

    Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com