Overview

  • Product name

    Anti-Mumps nucleoprotein antibody [8H4]
    See all Mumps nucleoprotein primary antibodies
  • Description

    Mouse monoclonal [8H4] to Mumps nucleoprotein
  • Host species

    Mouse
  • Specificity

    Recognises recombinant yeast-derived mumps NP. Recognises native NP in mumps virus infected Vero cells. This antibody has been tested on 3 different mumps virus strains isolated in the UK: 55799-98, SJ801-95 and Gloucester.
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • General notes

    This product was changed from ascites to tissue culture supernatant on 28/11/2017. Please note that the dilutions may need to be adjusted accordingly.

Properties

Applications

Our Abpromise guarantee covers the use of ab9876 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1 - 1/20.

Target

Images

  • Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.



    Immunofluorescence staining of mumps virus infected Vero cells with monoclonal antibody 8H4.

References

This product has been referenced in:

  • Wu H  et al. Mouse Testicular Cell Type-Specific Antiviral Response against Mumps Virus Replication. Front Immunol 8:117 (2017). Read more (PubMed: 28239382) »
  • Jiang Q  et al. C-X-C motif chemokine ligand 10 produced by mouse Sertoli cells in response to mumps virus infection induces male germ cell apoptosis. Cell Death Dis 8:e3146 (2017). Mouse . Read more (PubMed: 29072682) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your recent telephone enquiry. I can confirm that ab9879,ab9880 and ab9392 have been created using nuclear protein from Recombinant mumps NP.Gloucester strain (Glouc 1/UK96). Antibodies ab9876 and ab9878 have been raised using recombiant NP protein. I am unable to find data on which strain this is taken from. I can confirm all of these antibodies (ab9392,ab9876, ab9878, ab9879, ab9880) have also been succesfully tested in mumps strains 55799-98 and SJ801-95. I can suggest checking the sequence alignment of the NP protein of the different strains you are interested in. We recommned that sequence homology should be over 85% to predict an antibody will detect a protein. Mumps antibody ab20859 has been tested sucessfully on the Enders strain (from which the immunogen was taken) and a CDC strain #V5-004. Other strains (including 55799-98 and SJ801-95 and Gloucester) have not been tested, including 55799-98 and SJ801-95 and Gloucester. Again, I can suggest conducting a sequence alignment search to see which strains may be detectable. I hope this information is helpful. Should you require any further assistance, please do not hesitate to contact us again.

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