Recombinant
RabMAb

Recombinant Anti-Munc 13-4 antibody [EPR4914] (ab109113)

Overview

  • Product name

    Anti-Munc 13-4 antibody [EPR4914]
    See all Munc 13-4 primary antibodies
  • Description

    Rabbit monoclonal [EPR4914] to Munc 13-4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from a region of human Munc 13-4 (Q70J99).

  • Positive control

    • A673, K562, HepG2, Molt-4, and RAW264.7.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4914
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
    • Tissue specificity

      Expressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
    • Involvement in disease

      Defects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
    • Sequence similarities

      Belongs to the unc-13 family.
      Contains 2 C2 domains.
      Contains 1 MHD1 (MUNC13 homology domain 1) domain.
      Contains 1 MHD2 (MUNC13 homology domain 2) domain.
    • Domain

      The MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
    • Cellular localization

      Cytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
    • Information by UniProt
    • Database links

    • Form

      There are 3 isoforms produced by alternative splicing.
    • Alternative names

      • FHL 3 antibody
      • FHL3 antibody
      • FLJ00067 antibody
      • HLH 3 antibody
      • HLH3 antibody
      • HPLH 3 antibody
      • HPLH3 antibody
      • Jinx antibody
      • Munc13 4 antibody
      • Munc13-4 antibody
      • Protein unc 13 homolog D antibody
      • Protein unc-13 homolog D antibody
      • UN13D_HUMAN antibody
      • Unc 13 homolog D antibody
      • UNC 13D antibody
      • Unc-13 homolog D (C. elegans) antibody
      • Unc13 homolog D (C elegans) antibody
      • Unc13 homolog D antibody
      • UNC13, C. elegans, homolog of, D antibody
      • UNC13D antibody
      • Unc13h4 antibody
      see all

    Images

    • All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Molt-4 cell lysate
      Lane 5 : RAW264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 123 kDa

    • Flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.

    References

    ab109113 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Answer

    Thank you for contacting us regarding Ab109113.

    The Flow Cytometry data shown on our webpage usedthe following secondary antibody from JacksonImmuno Research:

    Code Number: 111-095-144 Description:
    Fluorescein (FITC) AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms,and Rat Sr Prot)



    We use our standard recommended Flow Cytometry protocol for our data. I have included a link to that protocol for your review should you desire:

    https://www.abcam.com/index.html?pageconfig=resource&rid=11381


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up