Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4914] to Munc 13-4
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
Product nameAnti-Munc 13-4 antibody [EPR4914]
See all Munc 13-4 primary antibodies
DescriptionRabbit monoclonal [EPR4914] to Munc 13-4
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide from a region of human Munc 13-4 (Q70J99).
- WB: A673, K562, HepG2, Molt-4, and RAW264.7. Flow Cyt: Molt-4.
We have preliminary internal testing data to indicate the Munc 13-4 antibody (ab109113) may not react with rat. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 123 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionPlays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse. Regulates assembly of recycling and late endosomal structures, leading to the formation of an endosomal exocytic compartment that fuses with perforin-containing granules at the immunologic synapse and licences them for exocytosis. Regulates Ca(2+)-dependent secretory lysosome exocytosis in mast cells.
Tissue specificityExpressed at high levels in spleen, thymus and leukocytes. Also expressed in lung and placenta, and at very low levels in brain, heart, skeletal muscle and kidney. Expressed in cytotoxic T-lymphocytes (CTL) and mast cells.
Involvement in diseaseDefects in UNC13D are the cause of hemophagocytic lymphohistiocytosis familial type 3 (FHL3) [MIM:608898]; also known as HPLH3. Familial hemophagocytic lymphohistiocytosis (FHL) is a genetically heterogeneous, rare autosomal recessive disorder. It is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. The clinical features of the disease include fever, hepatosplenomegaly, cytopenia, hypertriglyceridemia, hypofibrinogenemia, and neurological abnormalities ranging from irritability and hypotonia to seizures, cranial nerve deficits, and ataxia. Hemophagocytosis is a prominent feature of the disease, and a non-malignant infiltration of macrophages and activated T lymphocytes in lymph nodes, spleen, and other organs is also found.
Sequence similaritiesBelongs to the unc-13 family.
Contains 2 C2 domains.
Contains 1 MHD1 (MUNC13 homology domain 1) domain.
Contains 1 MHD2 (MUNC13 homology domain 2) domain.
DomainThe MHD1 and MHD2 domains mediate localization on recycling endosomes and lysosome.
Cellular localizationCytoplasm. Membrane. Late endosome. Recycling endosome. Lysosome. Colocalizes with cytotoxic granules at the plasma membrane. Localizes to endosomal exocytic vesicles.
- Information by UniProt
FormThere are 3 isoforms produced by alternative splicing.
- FHL 3 antibody
- FHL3 antibody
- FLJ00067 antibody
All lanes : Anti-Munc 13-4 antibody [EPR4914] (ab109113) at 1/1000 dilution
Lane 1 : A673 cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Molt-4 cell lysate
Lane 5 : RAW264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 123 kDa
Flow cytometric analysis of permeabilized Molt-4 cells using ab109113 in red.
ab109113 has been referenced in 1 publication.
- Xu R et al. Munc13-4 mediates human neutrophil elastase-induced airway mucin5AC hypersecretion by interacting with syntaxin2. Mol Med Rep 18:1015-1024 (2018). PubMed: 29767240