Key features and details
- Sheep polyclonal to Munc13-1 - C-terminal
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Munc13-1 antibody - C-terminal
See all Munc13-1 primary antibodies
DescriptionSheep polyclonal to Munc13-1 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Human
Recombinant fragment corresponding to Human Munc13-1 aa 1525-1698 (C terminal). (Derived from E.coli).
DPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIEVNII GPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDY CFAREDRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQ RSNDEVAKEFVKLKSDTRSAEEGG
Database link: Q9UPW8
- Mouse embryonic tissue lysate.
Previously labelled as Munc 13.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe IgG fraction of immunized serum was purified by antigen affinity chromatography.
Our Abpromise guarantee covers the use of ab215426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 190-210 kDa (predicted molecular weight: 193 kDa).|
RelevanceFunction: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity-dependent refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in most excitatory/glutamatergic but not inhibitory/GABA-mediated synapses (By similarity). Also involved in secretory granule priming in insulin secretion (By similarity). Interacts with FBXO45 (via SRY domain); leading to the degradation of UNC13A by the proteasome (By similarity). Plays a role in dendrite formation by melanocytes (PubMed:23999003). Tissue specificity: Expressed in pancreatic islet cells (PubMed:12871971). Expressed in melanocytes (PubMed:23999003). Similarity: Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger. Domain: The C2 domains are not involved in calcium-dependent phospholipid binding. The C-terminal region containing both MHD domains and the third C2 domain is required for synaptic vesicle priming activity.
Cellular localizationCytoplasm. Cell membrane; Peripheral membrane protein. Cell junction; synapse, presynaptic cell membrane. Note: Localized to the active zone of presynaptic density. Translocated to the plasma membrane as response to phorbol ester binding
- KIAA1032 antibody
- Munc 13 antibody
- Munc13-1 antibody
Anti-Munc13-1 antibody - C-terminal (ab215426) at 1/1000 dilution + Mouse embryonic tissue lysate
Predicted band size: 193 kDa
Observed band size: 190-210 kDa why is the actual band size different from the predicted?
ab215426 has not yet been referenced specifically in any publications.