Overview

  • Product name
  • Description
    Rabbit polyclonal to Munc18-1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Xenopus laevis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 354-574 of Human Munc18.

  • Positive control
    • 293T, A431, Jurkat, Raji and mouse brain lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab155020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • Tissue specificity
    Brain and spinal cord. Highly enriched in axons.
  • Involvement in disease
    Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
  • Sequence similarities
    Belongs to the STXBP/unc-18/SEC1 family.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ37475 antibody
    • Munc 18 1 antibody
    • Munc 18a antibody
    • MUNC18 1 antibody
    • N-Sec1 antibody
    • Neuronal SEC1 antibody
    • NSec1 antibody
    • p67 antibody
    • Protein unc-18 homolog 1 antibody
    • Protein unc-18 homolog A antibody
    • Rb sec1 antibody
    • RBSEC1 antibody
    • STXB1_HUMAN antibody
    • STXBP1 antibody
    • Syntaxin binding protein 1 antibody
    • Syntaxin-binding protein 1 antibody
    • Unc 18 homolog antibody
    • Unc 18A antibody
    • Unc-18A antibody
    • Unc18 1 antibody
    • UNC18 antibody
    • Unc18-1 antibody
    see all

Images

  • All lanes : Anti-Munc18-1 antibody (ab155020) at 1/3000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Anti-Munc18-1 antibody (ab155020) at 1/10000 dilution + mouse brain whole cell lysate at 20 µg

    Predicted band size: 68 kDa



    7.5% SDS PAGE
  • Confocal immunofluorescent analysis of methanol-fixed A431 cells labeling Munc18 with ab155020 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).

References

ab155020 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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