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    munc18-1-antibody-ab226372.pdf

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Neuroscience Neurotransmission Secretory Vesicles Munc18
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Anti-Munc18-1 antibody (ab226372)

  • Datasheet
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Western blot - Anti-Munc18-1 antibody (ab226372)
  • Immunoprecipitation - Anti-Munc18-1 antibody (ab226372)

Key features and details

  • Rabbit polyclonal to Munc18-1
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Munc18-1 antibody
    See all Munc18-1 primary antibodies
  • Description

    Rabbit polyclonal to Munc18-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Giant Panda
  • Immunogen

    Synthetic peptide within Human Munc18-1 aa 150-200. The exact sequence is proprietary.
    Database link: P61764

  • Positive control

    • IP: HeLa whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab226372 was affinity purified using an epitope specific to Munc18-1 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Munc18
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab30128)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)

Applications

Our Abpromise guarantee covers the use of ab226372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 68,69 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • Tissue specificity

    Brain and spinal cord. Highly enriched in axons.
  • Involvement in disease

    Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
  • Sequence similarities

    Belongs to the STXBP/unc-18/SEC1 family.
  • Cellular localization

    Cytoplasm. Membrane.
  • Target information above from: UniProt accession P61764 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 404293 Chicken
    • Entrez Gene: 282378 Cow
    • Entrez Gene: 609241 Dog
    • Entrez Gene: 6812 Human
    • Entrez Gene: 20910 Mouse
    • Entrez Gene: 100173563 Orangutan
    • Entrez Gene: 25558 Rat
    • Entrez Gene: 101103306 Sheep
    • Omim: 602926 Human
    • SwissProt: Q6R748 Chicken
    • SwissProt: P61763 Cow
    • SwissProt: P61764 Human
    • SwissProt: O08599 Mouse
    • SwissProt: Q5R6D2 Orangutan
    • SwissProt: P61765 Rat
    • Unigene: 288229 Human
    • Unigene: 278865 Mouse
    • Unigene: 80843 Rat
    see all
  • Alternative names

    • FLJ37475 antibody
    • Munc 18 1 antibody
    • Munc 18a antibody
    • MUNC18 1 antibody
    • N-Sec1 antibody
    • Neuronal SEC1 antibody
    • NSec1 antibody
    • p67 antibody
    • Protein unc-18 homolog 1 antibody
    • Protein unc-18 homolog A antibody
    • Rb sec1 antibody
    • RBSEC1 antibody
    • STXB1_HUMAN antibody
    • STXBP1 antibody
    • Syntaxin binding protein 1 antibody
    • Syntaxin-binding protein 1 antibody
    • Unc 18 homolog antibody
    • Unc 18A antibody
    • Unc-18A antibody
    • Unc18 1 antibody
    • UNC18 antibody
    • Unc18-1 antibody
    see all

Images

  • Western blot - Anti-Munc18-1 antibody (ab226372)
    Western blot - Anti-Munc18-1 antibody (ab226372)
    All lanes : Anti-Munc18-1 antibody (ab226372) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 68,69 kDa


    Exposure time: 30 seconds


    Cell lysates were prepared with NETN lysis buffer.

  • Immunoprecipitation - Anti-Munc18-1 antibody (ab226372)
    Immunoprecipitation - Anti-Munc18-1 antibody (ab226372)

    Munc18-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226372 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226372 at 1 µg/ml.

    Lane 1: ab226372 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 minutes.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab226372? Please let us know so that we can cite the reference in this datasheet.

    ab226372 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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