Anti-Munc18-1 antibody (ab226372)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Munc18-1 antibody
See all Munc18-1 primary antibodies -
Description
Rabbit polyclonal to Munc18-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Chicken, Cow, Cat, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Giant Panda
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Immunogen
Synthetic peptide within Human Munc18-1 aa 150-200. The exact sequence is proprietary.
Database link: P61764 -
Positive control
- IP: HeLa whole cell lysate. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226372 was affinity purified using an epitope specific to Munc18-1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 68,69 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions. -
Tissue specificity
Brain and spinal cord. Highly enriched in axons. -
Involvement in disease
Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination. -
Sequence similarities
Belongs to the STXBP/unc-18/SEC1 family. -
Cellular localization
Cytoplasm. Membrane. - Information by UniProt
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Database links
- Entrez Gene: 404293 Chicken
- Entrez Gene: 282378 Cow
- Entrez Gene: 609241 Dog
- Entrez Gene: 6812 Human
- Entrez Gene: 20910 Mouse
- Entrez Gene: 100173563 Orangutan
- Entrez Gene: 25558 Rat
- Entrez Gene: 101103306 Sheep
see all -
Alternative names
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
see all
Images
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Western blot - Anti-Munc18-1 antibody (ab226372)All lanes : Anti-Munc18-1 antibody (ab226372) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 68,69 kDa
Exposure time: 30 secondsCell lysates were prepared with NETN lysis buffer.
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Immunoprecipitation - Anti-Munc18-1 antibody (ab226372)Munc18-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using NETN lysis buffer; 20% of IP loaded) with ab226372 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226372 at 1 µg/ml.
Lane 1: ab226372 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Datasheets and documents
References
ab226372 has not yet been referenced specifically in any publications.
