Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Munc18-1 antibody
See all Munc18-1 primary antibodies
DescriptionRabbit polyclonal to Munc18-1
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken, Cow, Xenopus laevis, Rhesus monkey
Recombinant fragment within Human Munc18-1 (internal sequence). The exact sequence is proprietary.
Database link: P61764
- IHC-P: U373 xenograft tissue. WB: A431 whole cell lysate; Mouse and rat brain tissue extract. ICC/IF: DIV9 rat E18 primary cortical neurons.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/500 - 1/20000. Predicted molecular weight: 68 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
Tissue specificityBrain and spinal cord. Highly enriched in axons.
Involvement in diseaseDefects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
All lanes : Anti-Munc18-1 antibody (ab227971) at 1/10000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 68 kDa
7.5% SDS-PAGE gel.
4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Munc18-1 (green) using ab227971 at 1/500 dilution in ICC/IF.
Nuclear counter stain: DAPI (blue). beta tubulin 3/Tuj1 was stained using a beta Tubulin 3/ Tuj1 antibody (red).
Anti-Munc18-1 antibody (ab227971) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 68 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded U373 xenograft tissue stained for Munc18-1 using ab227971 at 1/500 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227971 has not yet been referenced specifically in any publications.