Overview

  • Product name

  • Description

    Rabbit polyclonal to Munc18-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Cow, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Munc18-1 (internal sequence). The exact sequence is proprietary.
    Database link: P61764

  • Positive control

    • IHC-P: U373 xenograft tissue. WB: A431 whole cell lysate; Mouse and rat brain tissue extract. ICC/IF: DIV9 rat E18 primary cortical neurons.

Properties

Applications

Our Abpromise guarantee covers the use of ab227971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/20000. Predicted molecular weight: 68 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • Tissue specificity

    Brain and spinal cord. Highly enriched in axons.
  • Involvement in disease

    Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
  • Sequence similarities

    Belongs to the STXBP/unc-18/SEC1 family.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37475 antibody
    • Munc 18 1 antibody
    • Munc 18a antibody
    • MUNC18 1 antibody
    • N-Sec1 antibody
    • Neuronal SEC1 antibody
    • NSec1 antibody
    • p67 antibody
    • Protein unc-18 homolog 1 antibody
    • Protein unc-18 homolog A antibody
    • Rb sec1 antibody
    • RBSEC1 antibody
    • STXB1_HUMAN antibody
    • STXBP1 antibody
    • Syntaxin binding protein 1 antibody
    • Syntaxin-binding protein 1 antibody
    • Unc 18 homolog antibody
    • Unc 18A antibody
    • Unc-18A antibody
    • Unc18 1 antibody
    • UNC18 antibody
    • Unc18-1 antibody
    see all

Images

  • All lanes : Anti-Munc18-1 antibody (ab227971) at 1/10000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Munc18-1 (green) using ab227971 at 1/500 dilution in ICC/IF.

    Nuclear counter stain: DAPI (blue). beta tubulin 3/Tuj1 was stained using a beta Tubulin 3/ Tuj1 antibody (red).

  • Anti-Munc18-1 antibody (ab227971) at 1/1000 dilution + A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

    Predicted band size: 68 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded U373 xenograft tissue stained for Munc18-1 using ab227971 at 1/500 dilution in immunohistochemical analysis.

References

ab227971 has not yet been referenced specifically in any publications.

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