Key features and details
- Rabbit polyclonal to Munc18-1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Munc18-1 antibody
See all Munc18-1 primary antibodies
DescriptionRabbit polyclonal to Munc18-1
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Orangutan
Synthetic peptide within Human Munc18-1 aa 544-594. The exact sequence is proprietary.
Database link: P61764
- IP: HeLa whole cell lysate.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Use at 2-10 µg/mg of lysate.
FunctionMay participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
Tissue specificityBrain and spinal cord. Highly enriched in axons.
Involvement in diseaseDefects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
Munc18-1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) with ab264385 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab264385 at 1 µg/ml.
Lane 1: ab264385 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with an exposure time of 30 seconds.
Lysates prepared using NETN lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264385 has not yet been referenced specifically in any publications.