• Product name

  • Description

    Rabbit polyclonal to Munc18-1
  • Host species

  • Tested applications

    Suitable for: IHC-Fr, IHC-FoFr, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Dog
    Predicted to work with: Chicken, Cow, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Munc18-1 aa 58-70.


    (Peptide available as ab4982)

  • Positive control

    • Rat whole brain extract.



Our Abpromise guarantee covers the use of ab3451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 23209433
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 67 kDa.

By Western blot, this antibody detects an ~67 kDa protein representing Munc-18 from rat whole brain extract.

ICC/IF Use at an assay dependent concentration. PubMed: 18487653


  • Function

    May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
  • Tissue specificity

    Brain and spinal cord. Highly enriched in axons.
  • Involvement in disease

    Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
  • Sequence similarities

    Belongs to the STXBP/unc-18/SEC1 family.
  • Cellular localization

    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ37475 antibody
    • Munc 18 1 antibody
    • Munc 18a antibody
    • MUNC18 1 antibody
    • N-Sec1 antibody
    • Neuronal SEC1 antibody
    • NSec1 antibody
    • p67 antibody
    • Protein unc-18 homolog 1 antibody
    • Protein unc-18 homolog A antibody
    • Rb sec1 antibody
    • RBSEC1 antibody
    • STXB1_HUMAN antibody
    • STXBP1 antibody
    • Syntaxin binding protein 1 antibody
    • Syntaxin-binding protein 1 antibody
    • Unc 18 homolog antibody
    • Unc 18A antibody
    • Unc-18A antibody
    • Unc18 1 antibody
    • UNC18 antibody
    • Unc18-1 antibody
    see all


  • Anti-Munc18-1 antibody (ab3451) at 1/5000 dilution + Mouse hippocampal homogenate at 5 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97069) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 67 kDa
    why is the actual band size different from the predicted?

    Exposure time: 1 minute

    See Abreview

  • Western blot of Munc-18 in rat brain homogenate immunoprecipitated with ab3451.
    1. Extract only.
    2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of ab3451, respectively.

  • ICC/IF image of ab3451 stained PC12 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3451, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemical analysis of PFA-perfusion fixed mouse brain tissue, staining Munc18-1 with ab3451.

    Tissue was post-fixed in 4% paraformaldehyde overnight at 4°C. Sections were incubated in a blocking buffer of 0.3% Triton-X-100, 1% BSA and 10% NGS in PBS for 2 hours at room temperature, before incubating with primary antibody (1/100) for 2 days at 4°C. An AlexaFluor®-conjugated anti-rabbit IgG (1/1000) was used as the secondary antibody.


This product has been referenced in:

  • Henderson BW  et al. Rho-associated protein kinase 1 (ROCK1) is increased in Alzheimer's disease and ROCK1 depletion reduces amyloid-ß levels in brain. J Neurochem 138:525-31 (2016). Read more (PubMed: 27246255) »
  • Wagnon JL  et al. CELF4 regulates translation and local abundance of a vast set of mRNAs, including genes associated with regulation of synaptic function. PLoS Genet 8:e1003067 (2012). WB, IHC-FoFr ; Mouse . Read more (PubMed: 23209433) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Mouse Tissue lysate - whole (Hippocampal homogenate)
Hippocampal homogenate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 12 2014

Western blot
Mouse Tissue lysate - whole (Hippocampus)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% TGS)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Aug 20 2008

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