Key features and details
- Goat polyclonal to Munc18-1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Munc18-1 antibody
See all Munc18-1 primary antibodies
DescriptionGoat polyclonal to Munc18-1
SpecificityThis antibody is expected to recognize isoform a (NP_003156.1).
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
- WB: Human brain tissue lysate. IHC-P: Human cortex tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab52011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 5 - 10 µg/ml.|
FunctionMay participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
Tissue specificityBrain and spinal cord. Highly enriched in axons.
Involvement in diseaseDefects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
Sequence similaritiesBelongs to the STXBP/unc-18/SEC1 family.
Cellular localizationCytoplasm. Membrane.
- Information by UniProt
- FLJ37475 antibody
- Munc 18 1 antibody
- Munc 18a antibody
Anti-Munc18-1 antibody (ab52011) at 0.1 µg/ml + Human Brain lysate (35µg protein in RIPA buffer)
Predicted band size: 68 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab52011 at 5 µg/ml staining Munc18 in Human cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab52011 has not yet been referenced specifically in any publications.