• Product name

    Anti-Munc18-1 antibody [EPR4850]
    See all Munc18-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4850] to Munc18-1
  • Host species

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Munc18-1 aa 450-550. The exact sequence is proprietary.

  • Positive control

    • SH-SY5Y, HeLa, Jurkat and Y79 cell lysates; Human brain tissue.
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124920 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      May participate in the regulation of synaptic vesicle docking and fusion, possibly through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. May play a role in determining the specificity of intracellular fusion reactions.
    • Tissue specificity

      Brain and spinal cord. Highly enriched in axons.
    • Involvement in disease

      Defects in STXBP1 are the cause of epileptic encephalopathy early infantile type 4 (EIEE4) [MIM:612164]. Affected individuals have neonatal or infantile onset of seizures, suppression-burst pattern on EEG, profound mental retardation, and MRI evidence of hypomyelination.
    • Sequence similarities

      Belongs to the STXBP/unc-18/SEC1 family.
    • Cellular localization

      Cytoplasm. Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ37475 antibody
      • Munc 18 1 antibody
      • Munc 18a antibody
      • MUNC18 1 antibody
      • N-Sec1 antibody
      • Neuronal SEC1 antibody
      • NSec1 antibody
      • p67 antibody
      • Protein unc-18 homolog 1 antibody
      • Protein unc-18 homolog A antibody
      • Rb sec1 antibody
      • RBSEC1 antibody
      • STXB1_HUMAN antibody
      • STXBP1 antibody
      • Syntaxin binding protein 1 antibody
      • Syntaxin-binding protein 1 antibody
      • Unc 18 homolog antibody
      • Unc 18A antibody
      • Unc-18A antibody
      • Unc18 1 antibody
      • UNC18 antibody
      • Unc18-1 antibody
      see all


    • All lanes : Anti-Munc18-1 antibody [EPR4850] (ab124920) at 1/10000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 68 kDa

    • ab124920, at 1/250 dilution, staining Munc18 in paraffin embedded Human brain tissue by immunohistochemistry

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


    This product has been referenced in:

    • Zhou J  et al. Quantitative Proteomic Analysis Reveals Molecular Adaptations in the Hippocampal Synaptic Active Zone of Chronic Mild Stress-Unsusceptible Rats. Int J Neuropsychopharmacol 19:N/A (2015). Read more (PubMed: 26364272) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Western blot
    Loading amount
    4 µg
    Gel Running Conditions
    Non-reduced Denaturing
    Rat Tissue lysate - whole (hippocampus tissue lysate)
    hippocampus tissue lysate
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C

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    Verified customer

    Submitted Jul 07 2014

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