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    munc18c-antibody-epr12447-ab175238.pdf

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Neuroscience Neurotransmission Secretory Vesicles Munc18
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RecombinantRabMAb

Recombinant Anti-Munc18c antibody [EPR12447] (ab175238)

  • Datasheet
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Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Anti-Munc18c antibody [EPR12447] (ab175238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12447] to Munc18c
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Munc18c antibody [EPR12447]
    See all Munc18c primary antibodies
  • Description

    Rabbit monoclonal [EPR12447] to Munc18c
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, Human spleen and K562 lysates; K562 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12447
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Munc18
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs

Associated products

  • Alternative Versions

    • Anti-Munc18c antibody [EPR12447] - BSA and Azide free (ab249886)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • K-562 whole cell lysate (ab7911)
  • Recombinant Protein

    • Recombinant Human Munc18c protein (ab159609)
  • Related Products

    • Recombinant Human Munc18c protein (ab159609)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.
  • Tissue specificity

    Megakaryocytes and platelets.
  • Sequence similarities

    Belongs to the STXBP/unc-18/SEC1 family.
  • Post-translational
    modifications

    Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4.
  • Cellular localization

    Cytoplasm > cytosol. Cell membrane. In platelets, predominantly cytosolic. Low amounts membrane-associated.
  • Target information above from: UniProt accession O00186 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6814 Human
    • Omim: 608339 Human
    • SwissProt: O00186 Human
    • Unigene: 530436 Human
    • Alternative names

      • Munc18 3 antibody
      • Munc18 c antibody
      • Platelet Sec1 protein antibody
      • Protein unc-18 homolog 3 antibody
      • Protein unc-18 homolog C antibody
      • PSP antibody
      • STXB3_HUMAN antibody
      • STXBP 3 antibody
      • STXBP3 antibody
      • Sxtbp3 antibody
      • syntaxin 4 binding protein antibody
      • Syntaxin binding protein 3 antibody
      • Syntaxin-binding protein 3 antibody
      • Unc 18 3 antibody
      • Unc 18 homolog 3 antibody
      • Unc 18c antibody
      • Unc-18C antibody
      • Unc18-3 antibody
      see all

    Images

    • Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
      Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
      All lanes : Anti-Munc18c antibody [EPR12447] (ab175238) at 1/10000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human spleen lysate
      Lane 3 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 68 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)
      Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)

      Immunofluorescent analysis of K562 cells labeling Munc18c with ab175238 at 1/100 (red) and DAPI staining (blue).

    • Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)
      Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)

      Immunoprecipitation of Munc18c from Human fetal kidney lysate using ab175238 at 1/10

    • Anti-Munc18c antibody [EPR12447] (ab175238)
      Anti-Munc18c antibody [EPR12447] (ab175238)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (1)

    Publishing research using ab175238? Please let us know so that we can cite the reference in this datasheet.

    ab175238 has been referenced in 1 publication.

    • Brasher MI  et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem 292:16199-16210 (2017). PubMed: 28798239

    Customer reviews and Q&As

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