Recombinant Anti-Munc18c antibody [EPR12447] (ab175238)
![Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.com/ps/products/175/ab175238/Images/ab175238-201748-anti-munc18c-antibody-epr12447-western-blot.jpg "Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12447] to Munc18c
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Munc18c antibody [EPR12447]
See all Munc18c primary antibodies -
Description
Rabbit monoclonal [EPR12447] to Munc18c -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, Human spleen and K562 lysates; K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12447 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab175238 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
|
|
| ICC/IF |
1/100 - 1/250.
|
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| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa. |
|
ICC/IF
1/100 - 1/250. |
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IP
1/10 - 1/100. |
Target
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Function
Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. -
Tissue specificity
Megakaryocytes and platelets. -
Sequence similarities
Belongs to the STXBP/unc-18/SEC1 family. -
Post-translational
modificationsPhosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4. -
Cellular localization
Cytoplasm > cytosol. Cell membrane. In platelets, predominantly cytosolic. Low amounts membrane-associated. - Information by UniProt
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Database links
- Entrez Gene: 6814 Human
- Omim: 608339 Human
- SwissProt: O00186 Human
- Unigene: 530436 Human
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Alternative names
- Munc18 3 antibody
- Munc18 c antibody
- Platelet Sec1 protein antibody
see all
Images
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Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)All lanes : Anti-Munc18c antibody [EPR12447] (ab175238) at 1/10000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human spleen lysate
Lane 3 : K562 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 68 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.com/ps/products/175/ab175238/Images/ab175238-201746-anti-munc18c-antibody-epr12447-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)Immunofluorescent analysis of K562 cells labeling Munc18c with ab175238 at 1/100 (red) and DAPI staining (blue).
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![Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.com/ps/products/175/ab175238/Images/ab175238-201747-anti-munc18c-antibody-epr12447-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)Immunoprecipitation of Munc18c from Human fetal kidney lysate using ab175238 at 1/10
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![Anti-Munc18c antibody [EPR12447] (ab175238)](https://www.abcam.com/ps/products/175/ab175238/Images/ab175238-4-benefits-of-recombinant-antibodies.png)
Anti-Munc18c antibody [EPR12447] (ab175238)
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab175238 has been referenced in 1 publication.
- Brasher MI et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem 292:16199-16210 (2017). PubMed: 28798239
