RecombinantRabMAb

Recombinant Anti-Munc18c antibody [EPR12447] (ab175238)

Submit a review Submit a question References (1)
Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)
  • Anti-Munc18c antibody [EPR12447] (ab175238)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12447] to Munc18c
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Munc18c antibody [EPR12447]
    See all Munc18c primary antibodies

  • Description

    Rabbit monoclonal [EPR12447] to Munc18c

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, Human spleen and K562 lysates; K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab175238 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 68 kDa.
ICC/IF
1/100 - 1/250.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Together with STX4 and VAMP2, may play a role in insulin-dependent movement of GLUT4 and in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes.

    • Tissue specificity

      Megakaryocytes and platelets.

    • Sequence similarities

      Belongs to the STXBP/unc-18/SEC1 family.

    • Post-translational
      modifications

      Phosphorylated by PKC in platelets in response to thrombin stimulation; phosphorylation inhibits binding to STX4.

    • Cellular localization

      Cytoplasm > cytosol. Cell membrane. In platelets, predominantly cytosolic. Low amounts membrane-associated.
    • Information by UniProt

    • Database links

      • Alternative names

        • Munc18 3 antibody
        • Munc18 c antibody
        • Platelet Sec1 protein antibody
        • Protein unc-18 homolog 3 antibody
        • Protein unc-18 homolog C antibody
        • PSP antibody
        • STXB3_HUMAN antibody
        • STXBP 3 antibody
        • STXBP3 antibody
        • Sxtbp3 antibody
        • syntaxin 4 binding protein antibody
        • Syntaxin binding protein 3 antibody
        • Syntaxin-binding protein 3 antibody
        • Unc 18 3 antibody
        • Unc 18 homolog 3 antibody
        • Unc 18c antibody
        • Unc-18C antibody
        • Unc18-3 antibody
        see all

      Images

      • Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
        Western blot - Anti-Munc18c antibody [EPR12447] (ab175238)
        All lanes : Anti-Munc18c antibody [EPR12447] (ab175238) at 1/10000 dilution

        Lane 1 : Human fetal kidney lysate
        Lane 2 : Human spleen lysate
        Lane 3 : K562 lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 68 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)
        Immunocytochemistry/ Immunofluorescence - Anti-Munc18c antibody [EPR12447] (ab175238)

        Immunofluorescent analysis of K562 cells labeling Munc18c with ab175238 at 1/100 (red) and DAPI staining (blue).

      • Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)
        Immunoprecipitation - Anti-Munc18c antibody [EPR12447] (ab175238)

        Immunoprecipitation of Munc18c from Human fetal kidney lysate using ab175238 at 1/10

      • Anti-Munc18c antibody [EPR12447] (ab175238)
        Anti-Munc18c antibody [EPR12447] (ab175238)

      References (1)

      Publishing research using ab175238? Please let us know so that we can cite the reference in this datasheet.

      ab175238 has been referenced in 1 publication.

      • Brasher MI  et al. Interaction of Munc18c and syntaxin4 facilitates invadopodium formation and extracellular matrix invasion of tumor cells. J Biol Chem 292:16199-16210 (2017). PubMed: 28798239

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab175238.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com