Anti-MURC antibody (ab121647)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-MURC antibody
See all MURC primary antibodies -
Description
Rabbit polyclonal to MURC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 95-180 (KVSAHIKDVK ARVEKQQIHV KKVEVKQEEI MKKNKFRVVI FQEKFRCPTS LSVVKDRNLT ENQEEDDDDI FDPPVDLSSD EEYYVE) of Human MURC.
-
Positive control
- Human skeletal muscle tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab121647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway. -
Sequence similarities
Belongs to the PTRF/SDPR family. -
Cellular localization
Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 347273 Human
- SwissProt: Q5BKX8 Human
- Unigene: 99004 Human
-
Alternative names
- murC antibody
- MURC_HUMAN antibody
- Muscle-related coiled-coil protein antibody
- Muscle-restricted coiled-coil protein antibody
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-MURC antibody (ab121647)Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MURC antibody (ab121647)ab121647, at 1/1000 dilution, staining MURC in paraffin-embedded Human skeletal muscle tissue, by Immunohistochemistry.
Datasheets and documents
References
ab121647 has not yet been referenced specifically in any publications.
