Anti-MURC antibody (ab121647)
Key features and details
- Rabbit polyclonal to MURC
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MURC antibody
See all MURC primary antibodies -
Description
Rabbit polyclonal to MURC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 95-180 (KVSAHIKDVK ARVEKQQIHV KKVEVKQEEI MKKNKFRVVI FQEKFRCPTS LSVVKDRNLT ENQEEDDDDI FDPPVDLSSD EEYYVE) of Human MURC.
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Positive control
- Human skeletal muscle tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab121647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
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| IHC-P | 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway. -
Sequence similarities
Belongs to the PTRF/SDPR family. -
Cellular localization
Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 347273 Human
- SwissProt: Q5BKX8 Human
- Unigene: 99004 Human
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Alternative names
- murC antibody
- MURC_HUMAN antibody
- Muscle-related coiled-coil protein antibody
- Muscle-restricted coiled-coil protein antibody
Images
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Immunocytochemistry/ Immunofluorescence - Anti-MURC antibody (ab121647)Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MURC antibody (ab121647)ab121647, at 1/1000 dilution, staining MURC in paraffin-embedded Human skeletal muscle tissue, by Immunohistochemistry.
Protocols
Datasheets and documents
References (0)
ab121647 has not yet been referenced specifically in any publications.
