Anti-MURC antibody (ab121647)
Key features and details
- Rabbit polyclonal to MURC
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MURC antibody
See all MURC primary antibodies -
Description
Rabbit polyclonal to MURC -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen, corresponding to amino acids 95-180 (KVSAHIKDVK ARVEKQQIHV KKVEVKQEEI MKKNKFRVVI FQEKFRCPTS LSVVKDRNLT ENQEEDDDDI FDPPVDLSSD EEYYVE) of Human MURC.
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Positive control
- Human skeletal muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment |
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 1 - 4 µg/ml. Recommend PFA Fixation and Triton X-100 treatment |
IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Induces RHOA activation and activates NPPA transcription and myofibrillar organization through the Rho/ROCK signaling pathway. -
Sequence similarities
Belongs to the PTRF/SDPR family. -
Cellular localization
Cytoplasm > myofibril > sarcomere. Cytoplasm. In cardiomyocytes, accumulates in the Z-line of the sarcomere. In vascular smooth muscle cells, detected diffusely throughout the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 347273 Human
- SwissProt: Q5BKX8 Human
- Unigene: 99004 Human
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Alternative names
- murC antibody
- MURC_HUMAN antibody
- Muscle-related coiled-coil protein antibody
- Muscle-restricted coiled-coil protein antibody
Images
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Immunofluorescent staining of Human cell line U-2 OS shows positivity in plasma membrane and cytoskeleton (microtubules). Recommended concentration of ab121647 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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ab121647, at 1/1000 dilution, staining MURC in paraffin-embedded Human skeletal muscle tissue, by Immunohistochemistry.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab121647 has been referenced in 1 publication.
- Tian Y et al. Integrated Bioinformatic Analysis of the Expression and Prognosis of Caveolae-Related Genes in Human Breast Cancer. Front Oncol 11:703501 (2021). PubMed: 34513683