Recombinant Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] -
Description
Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
Database link: Q969Q1 -
Positive control
- Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6431(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 40 kDa.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 40 kDa. |
Target
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Cellular localization
MURF1: Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. MURF3: Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Associates with microtubules. Localizes to the Z-lines in skeletal muscles. MURF2: Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules. -
Database links
- Entrez Gene: 57159 Human
- Entrez Gene: 84675 Human
- Entrez Gene: 84676 Human
- Entrez Gene: 381485 Mouse
- Entrez Gene: 433766 Mouse
- Entrez Gene: 58522 Mouse
- Entrez Gene: 140939 Rat
- Entrez Gene: 362708 Rat
see all
Images
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All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1 : Rat muscle tissue lysate
Lane 2 : Mouse muscle tissue lysate
Lane 3 : Human skeletal muscle tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 40 kDa -
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?
Exposure time: 180 seconds -
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 40 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 150 seconds -
Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 150 secondsBlocking and diluting buffer: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
References (53)
ab172479 has been referenced in 53 publications.
- Miyazaki M et al. Supplementing cultured human myotubes with hibernating bear serum results in increased protein content by modulating Akt/FOXO3a signaling. PLoS One 17:e0263085 (2022). PubMed: 35077510
- Ma X et al. Endoplasmic Reticulum Stress Is Involved in Muscular Pathogenesis in Idiopathic Inflammatory Myopathies. Front Cell Dev Biol 10:791986 (2022). PubMed: 35237595
- Duan J et al. Comparison of the effects of different calorie amounts of enteral nutrition in hypercatabolism associated with ghrelin-POMC in endotoxemic rats. Nutr Metab (Lond) 19:28 (2022). PubMed: 35428321
- Li S et al. Endoplasmic Reticulum Stress Contributes to Ventilator-Induced Diaphragm Atrophy and Weakness in Rats. Front Physiol 13:897559 (2022). PubMed: 35832486
- Testa MTJ et al. Resistance Training Attenuates Activation of STAT3 and Muscle Atrophy in Tumor-Bearing Mice. Front Oncol 12:880787 (2022). PubMed: 35847939