Overview

  • Product name
    Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)]
  • Description
    Rabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
    Database link: Q969Q1

  • Positive control
    • Rat muscle, Mouse muscle and Human skeletal muscle tissue lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab172479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 40 kDa.

Target

Images

  • All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate at 15 µg
    Lane 2 : Mouse muscle lysate at 20 µg

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution

    Lane 1 : Rat muscle tissue lysate
    Lane 2 : Mouse muscle tissue lysate
    Lane 3 : Human skeletal muscle tissue lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 40 kDa

  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF1 (aa 1 to 353) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution + His-Tagged human MURF3 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?


    Exposure time: 180 seconds
  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/500 dilution + His-Tagged human MURF2 (full length) recombinant protein at 1 µg with 5% NFDM/TBST

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

    Predicted band size: 40 kDa
    Observed band size: 75 kDa why is the actual band size different from the predicted?


    Exposure time: 150 seconds
  • Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/5000 dilution + Mouse muscle lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 40 kDa
    Observed band size: 40 kDa


    Exposure time: 150 seconds


    Blocking and diluting buffer: 5% NFDM/TBST

References

This product has been referenced in:
  • Wang F  et al. The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells. BMC Cancer 18:360 (2018). Read more (PubMed: 29609556) »
  • Onishi S  et al. Green tea extracts ameliorate high-fat diet-induced muscle atrophy in senescence-accelerated mouse prone-8 mice. PLoS One 13:e0195753 (2018). Read more (PubMed: 29630667) »
See all 15 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - other (muscle (cytosolic protein))
Gel Running Conditions
Reduced Denaturing (9)
Loading amount
50 µg
Specification
muscle (cytosolic protein)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Mr. Baptiste Jude

Verified customer

Submitted Oct 13 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up