Product nameAnti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] - BSA and Azide free
DescriptionRabbit monoclonal [EPR6431(2)] to MURF1 + MURF3 + MURF2 - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MURF1 aa 1-100. The exact sequence is proprietary.
Database link: Q969Q1
- WB: Human skeletal muscle lysate; Mouse muscle lysate.
Ab232364 is the carrier-free version of ab172479. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab232364 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab232364 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.|
Cellular localizationMURF1: Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes. MURF3: Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Associates with microtubules. Localizes to the Z-lines in skeletal muscles. MURF2: Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules.
All lanes : Anti-MURF1 + MURF3 + MURF2 antibody [EPR6431(2)] (ab172479) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Mouse muscle lysate
Lysates/proteins at 15 µg per lane.
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Blocking and diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab172479).
ab232364 has not yet been referenced specifically in any publications.