Key features and details
- Mouse monoclonal [7B8E1] to MURF1
- Suitable for: ICC/IF, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MURF1 antibody [7B8E1]
See all MURF1 primary antibodies
DescriptionMouse monoclonal [7B8E1] to MURF1
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
- HeLa cells; Human rectum cancer and muscle tissues.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Our Abpromise guarantee covers the use of ab201941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||1/200 - 1/1000.|
FunctionE3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Tissue specificityMuscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger.
Developmental stageExpressed throughout all developmental stages.
DomainThe RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
Cellular localizationCytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
- Information by UniProt
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
Immunofluorescent analysis of HeLa cells labeling MURF1 with ab201941 at 1/200 dilution. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
Flow cytometric analysis of HeLa cells labeling MURF1 with ab201941 at 1/200 dilution (green) compared to a negative control (red).
Immunohistochemical analysis of paraffin-embedded Human rectum cancer tissue labeling MURF1 with ab201941 at 1/200 dilution, with DAB staining.
Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling MURF1 with ab201941 at 1/200 dilution, with DAB staining.
ab201941 has been referenced in 1 publication.
- Kang SH et al. Forkhead box O3 plays a role in skeletal muscle atrophy through expression of E3 ubiquitin ligases MuRF-1 and atrogin-1 in Cushing's syndrome. Am J Physiol Endocrinol Metab 312:E495-E507 (2017). PubMed: 28246104