Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Cell Biology
- Proteolysis / Ubiquitin
- Proteasome / Ubiquitin
- Ubiquitin E3 Enzymes
- RING Finger E3 Ligase
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab4125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1.25 µg/ml.|
FunctionE3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Tissue specificityMuscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesContains 1 B box-type zinc finger.
Contains 1 COS domain.
Contains 1 RING-type zinc finger.
Developmental stageExpressed throughout all developmental stages.
DomainThe RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
Cellular localizationCytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
- Information by UniProt
- E3 ubiquitin-protein ligase TRIM63 antibody
- FLJ32380 antibody
- IRF antibody
ab4125 at 1.25 µg/ml staining human skeletal muscle by IHC-P. Tissue sections were subjected to steamed antigen retrieval with citrate buffer (pH6), AP-staining. Shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.
ab4125 staining MURF1 in mouse lung tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in paraformaldehyde and blocked in 1%BSA for 10 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with sample for 20 hours at 20°C. A Biotin conjugated rabbit polyclonal to goat IgG, diluted 1/500 was used as secondary.
This product has been referenced in:
- Zhu S et al. Mouse liver repopulation with hepatocytes generated from human fibroblasts. Nature 508:93-7 (2014). Read more (PubMed: 24572354) »
- Nedergaard A et al. Effects of 2 weeks lower limb immobilization and two separate rehabilitation regimens on gastrocnemius muscle protein turnover signaling and normalization genes. BMC Res Notes 5:166 (2012). Read more (PubMed: 22455386) »