Overview

  • Product name

  • Description

    Rabbit polyclonal to MURF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide containing a sequence corresponding to a region within amino acids 1-14 (

    MDYKSSLIQDGNPM

    ) of Human MURF1 (NP_115977).

  • Positive control

    • HepG2 whole cell lysate (ab7900); HeLa cells; 293T, A431, H1299, HeLaS3, Molt-4 and Raji cell lines

Properties

Applications

Our Abpromise guarantee covers the use of ab96857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
  • Tissue specificity

    Muscle specific. Selectively expressed in heart and skeletal muscle. Also expressed in the iris.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • Developmental stage

    Expressed throughout all developmental stages.
  • Domain

    The RING-type zinc finger mediates interaction with SUMO2 and localization to the nucleus. Also required for the E3 ubiquitin ligase activity.
  • Cellular localization

    Cytoplasm. Nucleus. Localizes to the M- and Z-lines in skeletal muscle. Colocalizes with TNNI3 in myocytes.
  • Information by UniProt
  • Database links

  • Alternative names

    • E3 ubiquitin-protein ligase TRIM63 antibody
    • FLJ32380 antibody
    • IRF antibody
    • Iris RING finger protein antibody
    • MURF 1 antibody
    • MURF-1 antibody
    • MuRF1 antibody
    • MURF2 antibody
    • Muscle specific ring finger protein 1 antibody
    • Muscle specific ring finger protein 2 antibody
    • Muscle-specific RING finger protein 1 antibody
    • OTTHUMP00000008701 antibody
    • RING finger protein 28 antibody
    • RNF 28 antibody
    • RNF28 antibody
    • SMRZ antibody
    • Striated muscle RING zinc finger protein antibody
    • TRI63_HUMAN antibody
    • TRIM 63 antibody
    • Trim63 antibody
    • Tripartite motif containing 63 antibody
    • tripartite motif containing 63, E3 ubiquitin protein ligase antibody
    • Tripartite motif containing protein 63 antibody
    • Tripartite motif-containing protein 63 antibody
    • Ubiquitin ligase TRIM63 antibody
    see all

Images

  • Anti-MURF1 antibody (ab96857) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 40 kDa



    10% SDS-PAGE
  • Immunofluorescence analysis of MURF1 in paraformaldehyde-fixed HeLa cells, using ab96857 at 1/200 dilution. Lower image is merged with DNA probe.

References

This product has been referenced in:

  • MacNeil LG  et al. The order of exercise during concurrent training for rehabilitation does not alter acute genetic expression, mitochondrial enzyme activity or improvements in muscle function. PLoS One 9:e109189 (2014). WB ; Human . Read more (PubMed: 25289940) »
  • Bailey AN  et al. MAFbx, MuRF1, and the stress-activated protein kinases are upregulated in muscle cells during total knee arthroplasty. Am J Physiol Regul Integr Comp Physiol 303:R376-86 (2012). Human . Read more (PubMed: 22761181) »
See all 2 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Sheep Tissue lysate - whole (skeletal muscle)
Gel Running Conditions
Non-reduced Denaturing (10)
Loading amount
50 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 23 2017

Answer

Thank you for contacting us.
Human Protein Reference Database shows that Murf1 may be expressed in liver tissue.
Please refer to:

http://www.hprd.org/summary?hprd_id=05843&isoform_id=05843_1&isoform_name=Isoform_1
Also, although most references indicate that Murf1 is muscle-specific, it may still be expressed at lower levels in other tissues.
The lab has confirmed that the HeLa and HepG2 lysates used to perform WB were cells expressing Murf1 protein, not transfected lysate.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More
Application
Western blot
Sample
Pig Tissue lysate - other (Porcine skeletal muscle (longissimus dorsi))
Loading amount
30 µg
Specification
Porcine skeletal muscle (longissimus dorsi)
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
StartingBlock (TBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jan 19 2012

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab96857 in swine. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forswine and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your interest in Abcam's MuRF1 antibodies. I have performed a search of our catalog and specifically looked at ab77577 and ab96857 for suitability with swine. Unfortunately ab77577 is out of stock long term, however I agree that ab96857 would be a suitable option. ab96857 is a rabbit polyclonal antibody whose immunogen shares 100% identity with swine. The antibody would be suitable for either WB or ICC/IF. Other applications have not been tested and are therefore not guaranteed under our Abpromise (https://www.abcam.com/index.html?pageconfig=abpromise). Although we cannot guarantee reactivity, I would like to offer a testing discount off a future purchase if you buy ab96857 now, test it in swine and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab96857 in swine. I will then send a discount code. This code must be issued before purchasing ab96857 so please wait for my reply before ordering. 2. Purchase ab96857 either by phone, fax, or online (www.abcam.com). 3. Test it in swine. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab96857 turns out to be unsuitable for swine, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m)
Loading amount
5 µg
Specification
Tibialis muscle of C57BL/6 and MuRF1/2 knock-out m
Treatment
LPS or NaCl as a control 48h
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris XT-Criterion BioRad)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 23 2011

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