Overview

  • Product name

    Anti-MURF2 antibody
  • Description

    Goat polyclonal to MURF2
  • Host species

    Goat
  • Tested applications

    Suitable for: WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide:

    HIFSFSWLNSLNE

    , corresponding to C terminal amino acids 536-546 of Human MURF2.

Properties

Applications

Our Abpromise guarantee covers the use of ab4387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/0.1 - 1/0.3. Detects a band of approximately 85 kDa (predicted molecular weight: 60 kDa). PubMed: 17272810
IHC-Fr Use at an assay dependent dilution.

Target

  • Function

    May regulate gene expression and protein turnover in muscle cells.
  • Tissue specificity

    Highly expressed in muscle. Low-level expression in liver.
  • Sequence similarities

    Contains 1 B box-type zinc finger.
    Contains 1 COS domain.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Cytoplasm. Nucleus. Nuclear under atrophic conditions and upon mechanical signals. Localizes to the sarcomeric M-band in cardiomyocytes. Colocalizes in part with microtubules.
  • Information by UniProt
  • Database links

  • Alternative names

    • MURF 2 antibody
    • MURF-2 antibody
    • MuRF2 antibody
    • Muscle specific ring finger 2 antibody
    • Muscle specific RING finger protein 2 antibody
    • Muscle-specific RING finger protein 2 antibody
    • RING finger protein 29 antibody
    • RNF 29 antibody
    • RNF29 antibody
    • TRI55_HUMAN antibody
    • TRIM 55 antibody
    • Trim55 antibody
    • Tripartite motif containing 55 antibody
    • Tripartite motif containing protein 55 antibody
    • Tripartite motif-containing protein 55 antibody
    see all

Images

  • Immunohistochemical analysis of Human prostate tissue labeling PBP2. Steamed antigen retrieval with citrate buffer pH 6.
  • Anti-MURF2 antibody (ab4387) at 0.1 µg/ml + Human heart lysate (in RIPA buffer) at 35 µg

    Predicted band size: 60 kDa

References

This product has been referenced in:

  • He J  et al. MuRF2 regulates PPAR?1 activity to protect against diabetic cardiomyopathy and enhance weight gain induced by a high fat diet. Cardiovasc Diabetol 14:97 (2015). WB . Read more (PubMed: 26242235) »
  • Cullup T  et al. Recessive mutations in EPG5 cause Vici syndrome, a multisystem disorder with defective autophagy. Nat Genet 45:83-7 (2013). Read more (PubMed: 23222957) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Muscle cell)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
60 µg
Specification
Muscle cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Mr. Joao de Oliveira Silvestre

Verified customer

Submitted Feb 11 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 18 2008

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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