Anti-Mus81 antibody [MTA30 2G10/3] (ab14387)
Key features and details
- Mouse monoclonal [MTA30 2G10/3] to Mus81
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Mus81 antibody [MTA30 2G10/3]
See all Mus81 primary antibodies -
Description
Mouse monoclonal [MTA30 2G10/3] to Mus81 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse -
Immunogen
Mus81 protein.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99.98% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
from TCS -
Clonality
Monoclonal -
Clone number
MTA30 2G10/3 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14387 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (7) |
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa).
Tested on both recombinant protein and whole cell extract. |
Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 66 kDa). Tested on both recombinant protein and whole cell extract. |
Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. May be required in mitosis for the processing of stalled or collapsed replication forks. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the XPF family.
Contains 1 ERCC4 domain. -
Developmental stage
Expressed in S phase and G2 phase. -
Cellular localization
Nucleus > nucleolus. Recruited to foci of DNA damage in S-phase cells. - Information by UniProt
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Database links
- Entrez Gene: 80198 Human
- Omim: 606591 Human
- SwissProt: Q96NY9 Human
- Unigene: 288798 Human
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Alternative names
- Crossover junction endonuclease MUS81 antibody
- FLJ21012 antibody
- FLJ44872 antibody
see all
Images
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Anti-Mus81 antibody [MTA30 2G10/3] (ab14387) at 1/1000 dilution + HeLa cell lysate
Predicted band size: 66 kDa
Observed band size: 64 kDa why is the actual band size different from the predicted? -
Overlay histogram showing HeLa cells stained with ab14387 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14387, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (55)
ab14387 has been referenced in 55 publications.
- Mehta KPM et al. CHK1 phosphorylates PRIMPOL to promote replication stress tolerance. Sci Adv 8:eabm0314 (2022). PubMed: 35353580
- Mocanu C et al. DNA replication is highly resilient and persistent under the challenge of mild replication stress. Cell Rep 39:110701 (2022). PubMed: 35443178
- Griffin WC et al. A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress. Nat Commun 13:5090 (2022). PubMed: 36042199
- Heijink AM et al. Sister chromatid exchanges induced by perturbed replication can form independently of BRCA1, BRCA2 and RAD51. Nat Commun 13:6722 (2022). PubMed: 36344511
- Said M et al. FANCD2 promotes mitotic rescue from transcription-mediated replication stress in SETX-deficient cancer cells. Commun Biol 5:1395 (2022). PubMed: 36543851