Anti-Musashi 1 / Msi1 antibody (ab218189)
Key features and details
- Rabbit polyclonal to Musashi 1 / Msi1
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-Musashi 1 / Msi1 antibody
See all Musashi 1 / Msi1 primary antibodies -
Description
Rabbit polyclonal to Musashi 1 / Msi1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Human -
Immunogen
Synthetic peptide within Human Musashi 1/ Msi1 aa 60-110 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O43347 -
Positive control
- IHC-P: Mouse brain and pancreas tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218189 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
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Notes |
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IHC-P
1/100 - 1/500. |
Target
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Function
RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. -
Tissue specificity
Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. -
Sequence similarities
Belongs to the Musashi family.
Contains 2 RRM (RNA recognition motif) domains. -
Domain
The first RNA recognition motif binds more strongly to RNA compared to the second one. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4440 Human
- Entrez Gene: 17690 Mouse
- Entrez Gene: 259272 Rat
- Omim: 603328 Human
- SwissProt: O43347 Human
- SwissProt: Q61474 Mouse
- SwissProt: Q8K3P4 Rat
- Unigene: 158311 Human
see all -
Alternative names
- Msi 1 antibody
- Msi1 antibody
- MSI1H_HUMAN antibody
see all
Images
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Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for Musashi 1 / Msi1 using ab218189 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
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Formalin-fixed, paraffin-embedded mouse brain tissue stained for Musashi 1 / Msi1 using ab218189 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab218189 has not yet been referenced specifically in any publications.