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  1. Link

    musashi-1--msi1-antibody-ep1302-ab52865.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

  • Datasheet
  • SDS
Reviews (18)Q&A (2)References (46)

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Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
  • Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
  • Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Chicken, Human, Quail

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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2,3-Butanedione 2-monoxime (BDM), Non-selective myosin ATPase inhibitor (ab120616)
Primary
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Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302] (ab199781)
Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Musashi 1 / Msi1 antibody [EP1302]
    See all Musashi 1 / Msi1 primary antibodies
  • Description

    Rabbit monoclonal [EP1302] to Musashi 1 / Msi1
  • Host species

    Rabbit
  • Specificity

    Several customers have found that this antibody gives good results in mouse and rat however in our hands, we cannot obtain positive results. This antibody is therefore no longer covered by our Abpromise guarantee for use in mouse or rat.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt (Intra), WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Chicken, Human, Quail
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human lung carcinoma tissue. Flow Cyt (intra): SH-SY5Y cells. ICC: SH-SY5Y, Neuro-2a and HAP1-MSI1 cells. WB: SH-SY5Y cell lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1302
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Nuclear
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Neural Stem Cells
    • Glial Restricted Lineage
    • Astrocyte

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Musashi 1 / Msi1 antibody [EP1302] (ab199781)
    • Alexa Fluor® 647 Anti-Musashi 1 / Msi1 antibody [EP1302] (ab199838)
    • PE Anti-Musashi 1 / Msi1 antibody [EP1302] (ab210418)
    • Anti-Musashi 1 / Msi1 antibody [EP1302] - BSA and Azide free (ab221797)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52865 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (7)
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/40.

WB (6)
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

For unpurified use at 1/2000.

Notes
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Flow Cyt (Intra)
1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  For unpurified use at 1/40.

WB
1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).

For unpurified use at 1/2000.

Application notes
Is unsuitable for IP.

Target

  • Function

    RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system.
  • Tissue specificity

    Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines.
  • Sequence similarities

    Belongs to the Musashi family.
    Contains 2 RRM (RNA recognition motif) domains.
  • Domain

    The first RNA recognition motif binds more strongly to RNA compared to the second one.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession O43347 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4440 Human
    • Entrez Gene: 17690 Mouse
    • Omim: 603328 Human
    • SwissProt: O43347 Human
    • SwissProt: Q61474 Mouse
    • Unigene: 158311 Human
    • Unigene: 5077 Mouse
    • Alternative names

      • Msi 1 antibody
      • Msi1 antibody
      • MSI1H_HUMAN antibody
      • Musashi homolog 1 antibody
      • Musashi RNA binding protein 1 antibody
      • Musashi-1 antibody
      • Musashi1 antibody
      • RNA binding protein Musashi homolog 1 antibody
      • RNA-binding protein Musashi homolog 1 antibody
      see all

    Images

    • Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Immunocytochemistry - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

      ab52865 staining Musashi 1 / Msi1 in HAP1-MSI1 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab52865 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.

    • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : MSI1 knockout HAP1 whole cell lysate
      Lane 3 : SH-SY5Y whole cell lysate

      Predicted band size: 39 kDa
      Observed band size: 39 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab52865 observed at 39 kDa. Red - loading control, ab130007, observed at 130 kDa.

      ab52865 was shown to specifically react with Musashi 1 / Msi1 in wild-type HAP1 cells as signal was lost in MSI1 knockout cells. Wild-type and MSI1 knockout samples were subjected to SDS-PAGE. Ab52865 and ab130007 (Mouse anti Vinculin loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      All lanes : Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/1000 dilution (purified)

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 2 : UMNSAH/DF-1 (chicken embryo fibroblast) whole cell lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 39 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

      Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with purified ab52865 at 1/80 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

      Immunocytochemistry/ Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:500 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200. Ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Mushashi 1/ Msi1 with Purified ab52865 at 1:50 dilution (17.7 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1:500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Immunocytochemistry/ Immunofluorescence - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

      Immunocytochemistry/Immunofluorescence analysis of Neuro-2a (mouse neuroblastoma) labelling Musashi 1 with purified ab52865 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Western blot - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865) at 1/2000 dilution (unpurified) + SH-SY-5Y cell lysate at 10 µg/ml

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 39 kDa
      Observed band size: 39 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)This image is courtesy of Carl Hobbs, King's College London, United Kingdom

      Immunohistochemistical detection (on formaldehyde/PFA-fixed paraffin-embedded sections) of Musashi 1 / Msi1 antibody [EP1302] (unpurified ab52865) on Quail Tissue sections (embryo d5/6 Brain stem T/S). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody unpurified ab52865 incubated at 1/300 for 2 hours at RT. Secondary Antibody: Biotin labelled goat anti rabbit IgG (1/300).

    • Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Flow Cytometry (Intracellular) - Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)This image is courtesy of an abreview submitted by Jennifer Moore, Stem Cell Research Center, United States

      Intracellular Intracellular Flow Cyt image of Musashi1 (ab52865)using Accutase digested single cell suspension of hESC (Neural stem cells derived from human embryonic). The cells were fixed and permeabilized . The cells were incubated with unpurified ab52865 (1/20 using Prem/wash solution) for 30 mins at 23°C. 

       

       

      See Abreview

    • Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)
      Anti-Musashi 1 / Msi1 antibody [EP1302] (ab52865)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (46)

    Publishing research using ab52865? Please let us know so that we can cite the reference in this datasheet.

    ab52865 has been referenced in 46 publications.

    • Baroni M  et al. Musashi1 Contribution to Glioblastoma Development via Regulation of a Network of DNA Replication, Cell Cycle and Division Genes. Cancers (Basel) 13:N/A (2021). PubMed: 33804958
    • Montalbano M  et al. RNA-binding proteins Musashi and tau soluble aggregates initiate nuclear dysfunction. Nat Commun 11:4305 (2020). PubMed: 32855391
    • Allensworth-James ML  et al. Metabolic signalling to somatotrophs: Transcriptional and post-transcriptional mediators. J Neuroendocrinol 32:e12883 (2020). PubMed: 32657474
    • Zhang K  et al. Imbalance of Excitatory/Inhibitory Neuron Differentiation in Neurodevelopmental Disorders with an NR2F1 Point Mutation. Cell Rep 31:107521 (2020). PubMed: 32320667
    • Maturi NP  et al. A molecularly distinct subset of glioblastoma requires serum-containing media to establish sustainable bona fide glioblastoma stem cell cultures. Glia 68:1228-1240 (2020). PubMed: 31868967
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 20 Abreviews or Q&A

    Flow Cytometry abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Differentiated hNSCs)
    Permeabilization
    Yes - 0.25% Triton X-100 in DPBS
    Gating Strategy
    Undifferentiated Stem Cells (white)
    Specification
    Differentiated hNSCs
    Preparation
    Cell harvesting/tissue preparation method: Accutase
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 15 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated
    Sample
    Human Tissue sections (Teratoma)
    Specification
    Teratoma
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    MR. Carl Hobbs

    Verified customer

    Submitted Feb 19 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (colon)
    Specification
    colon
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Leica Bond Epitope Retrieval 2
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Apteronotus leptorhynchus Tissue lysate - whole (Brain)
    Loading amount
    50 µg
    Specification
    Brain
    Gel Running Conditions
    Reduced Denaturing (4-15%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 29 2013

    Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (prostate cell)
    Loading amount
    50 µg
    Specification
    prostate cell
    Gel Running Conditions
    Reduced Denaturing (10 % gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 04 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Apteronotus leptorhynchus Tissue sections (Spinal cord)
    Specification
    Spinal cord
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.3% Triton X-100
    Blocking step
    3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Dr. Ruxandra Sirbulescu

    Verified customer

    Submitted Dec 08 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (human colon cancer tissue)
    Specification
    human colon cancer tissue
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer ph 6
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 36°C
    Read More

    Dr. Jaykumar Thumar

    Verified customer

    Submitted Dec 31 2010

    Flow Cytometry abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (Neural stem cells derived from human embryonic ste)
    Specification
    Neural stem cells derived from human embryonic ste
    Preparation
    Cell harvesting/tissue preparation method: Accutase digested single cell suspension of tissue culture cells
    Sample buffer: PBS
    Fixation
    BD Fixation/Permeabilization solution
    Permeabilization
    Yes - BD Fixation/Permeabilization solution
    Gating Strategy
    live cells
    Read More

    Dr. Jennifer Moore

    Verified customer

    Submitted Sep 06 2010

    Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Mouse Brain)
    Loading amount
    20 µg
    Specification
    Mouse Brain
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 17 2010

    Question

    Hi, I am wondering whether this musashi antibody is predicted to work in Xenopus laevis or Xenopus tropical is? I did noticed that 21628 is predicted to work in Xenopus laevis but is discouraged by the abreviews about 21628.

    Read More

    Abcam community

    Verified customer

    Asked on Feb 25 2013

    Answer

    Thank you very much for your interest our products.

    To our knowledge, neither ab21628 nor ab52865 have been tested in Xenopus. The homology of the immunogen of ab21628 with Xenopus laevis and tropicalis is 76%. The homology of the immunogen of ab52865 with Xenopus laevis and tropicalis is 80%. So actually, the homology with ab52865 is higher. We had incorrectly listed Xenopus as "predicted to react with" for ab21628, since 76% is quite low, so we thank you for bringing that to our attention.

    By participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing ab52865 in Xenopus.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial.

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    Abcam Scientific Support

    Answered on Feb 25 2013

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