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I have a couple of questions about the Musashi 1 Antibody (ab52865). Firstly, can you please forward me the literature that suggests PC12 cells as a positive control. Secondly, is there a recommended incubation period/time when using the antibody in immunofluorescent staining for cells grown in culture? Thank you,
Asked on Aug 17 2009
Thank you for your enquiry. I am afraid that using PC12 as a positive control information was not taken from any publication. The cell line was used in our laboratory testing and the results obtained were good. Therefore, we had that information stated on the datasheet as a positive control that customers can use. I did however manage to locate a publication on the subject on the internet, should you wish to refer. This publication reports Musashi expression in PC12 cells. Function and regulation of the mammalian Musashi mRNA translational regulator. Biochem Soc Trans. 2008 June; 36(Pt 3): 528–530. http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2562719 Our recommended incubation period and time for ICC/IF application is 1 hr room temperature, or overnight at 4 degrees. Please refer to our protocols page for more protocol details on the application. https://www.abcam.com/ps/pdf/protocols/icc.pdf I hope this information is helpful. If there is anything else that I can help you with, please do not hesitate to contact me.
Answered on Aug 17 2009