Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Brain)
Loading amount
20 µg
Specification
Mouse Brain
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 39 kDa

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Submitted Aug 06 2010

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