Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate cell)
Loading amount
50 µg
Specification
prostate cell
Gel Running Conditions
Reduced Denaturing (10 % gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/40000

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 37 kDa

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Submitted Jul 04 2012

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