Western blot abreview for Anti-Musashi 1 / Msi1 antibody [EP1302]

Inconclusive
Abreviews
Application
Western blot
Sample
Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount
50 µg
Specification
Brain
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 2.5% milk in PBS-Tween20

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Detection

Detection method
Fluorescence detection
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 150 kDa

Additional data

Additional Notes
This antibody recognizes specifically one band at approximately 150 kDa, possibly representing either an isoform or polymerized form of the target antigen, or a different protein.

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Submitted Jan 29 2013

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